Tracking the spatiotemporal dynamics of bacterial effectors injected into single living host cells has become possible with a split GFP–based method.
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Rodrigues, C., Enninga, J. The 'when and whereabouts' of injected pathogen effectors. Nat Methods 7, 267–269 (2010). https://doi.org/10.1038/nmeth0410-267
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DOI: https://doi.org/10.1038/nmeth0410-267