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Syntaxin 10: A Member of the Syntaxin Family Localized to theTrans-Golgi Network

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Abstract

We have cloned a new member of the syntaxin family of proteins, designated human syntaxin 10 (hsyn10). The open reading frame encodes a polypeptide of 249 amino acids with potential coiled-coil domains and a carboxy-terminal hydrophobic tail. hsyn10 is particularly homologous to the recently reported rat syntaxin 6 (about 60% identity). Northern blot analysis showed that the transcript is enriched in the heart, skeletal muscles and pancreas. Indirect immunofluorescence studies using polyclonal antibodies raised against recombinant protein showed that the protein is localized to intracellular membrane structures, with perinuclear staining patterns colocalising well with the Golgi SNARE GS28. Morphological alterations of the staining pattern of the protein with brefeldin A but not wortmannin treatment indicate that the protein is localize to the trans-Golgi network.

References (29)

  • S.F. Altschul et al.

    J. Mol. Biol.

    (1990)
  • C. Barlowe et al.

    Cell

    (1994)
  • M.K. Bennett et al.

    Cell

    (1993)
  • J.B. Bock et al.

    J. Biol. Chem.

    (1996)
  • J. Lippincott-Schwartz et al.

    Cell

    (1991)
  • J.E. Rothman et al.

    Curr. Biol.

    (1994)
  • T. Söllner et al.

    Cell

    (1993)
  • V.N. Subramaniam et al.

    J. Biol. Chem.

    (1992)
  • H. Wang et al.

    Gene

    (1997)
  • S.F. Altschul et al.

    Nucleic Acids Res.

    (1997)
  • F.M. Ausubel et al.

    Current Protocols in Molecular Biology

    (1993)
  • M.K. Bennett et al.

    Proc. Natl. Acad. Sci. (USA).

    (1993)
  • M.K. Bennett et al.

    Science

    (1992)
  • J.B. Bock et al.

    Nature

    (1997)
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    Abbreviations used: ER, endoplasmic reticulum; FBS, fetal bovine serum; FITC, fluorescein isothiocyanate; NSF, N-ethylmaleimide sensitive factor; RPMI medium, Rosewell Park Memorial Institute medium; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SNAP, soluble NSF attachment proteins, SNARE, SNAP receptor

    1

    The first two authors contributed equally.

    2

    Correspondence. Fax: (65) 779-1117. E-mail: [email protected].

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