Regular Article
Antiangiogenic Activity of Restin, NC10 Domain of Human Collagen XV: Comparison to Endostatin

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Abstract

Based on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic pET expression system. We have shown that restin inhibits the migration of endothelial cellsin vitrobut has no effect on the proliferation of these cells. A polyclonal antibody raised against endostatin cross-reacted with restin. Systemic administration of restin suppressed the growth of tumors in a xenograft renal carcinoma model.

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1

Equal contributions.

2

To whom correspondence should be addressed at Beth Israel Deaconess Medical Center, Dana 517, 330 Brookline Avenue, Boston, MA 02215. Fax: 617-667-7843. E-mail:[email protected].

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