Regular Article
High Levels of Ezrin Expressed by Human Pancreatic Adenocarcinoma Cell Lines with High Metastatic Potential

https://doi.org/10.1006/bbrc.1999.0653Get rights and content

Abstract

Ezrin is a membrane cytoskeleton crosslinker protein that is a member of the ERM (ezrin/radixin/moesin) family. Ezrin binds adhesion molecules such as CD43, CD44, ICAM-1, and ICAM-2, which are implicated in cell migration and metastasis. Ezrin is expressed by many tumor cell lines; however, little is known about the function of ezrin in tumorigenesis and metastasis. Here, we investigated expression of ezrin in pancreatic adenocarcinoma cell lines of different metastatic potential. Among 16 pancreatic adenocarcinoma cell lines, several cell lines showed strong expression of ezrin. Two cell lines with high metastatic potential, S2-CP9 and S2-VP10, showed very high levels of ezrin mRNA and protein, whereas other sublines showed lower levels. There was no relationship between the expression levels of ezrin and the differentiation grades of the cell lines. These results suggest that there is a relationship between high expression of ezrin and metastatic potential of pancreatic carcinomas.

References (26)

  • S. Tsukita et al.

    Curr. Opin. Cell. Biol.

    (1997)
  • A. Vaheri et al.

    Curr. Opin. Cell. Biol.

    (1997)
  • L. Heiska et al.

    J. Biol. Chem.

    (1998)
  • M. Arpin et al.

    Curr. Opin. Cell Biol.

    (1994)
  • U. Gunthert et al.

    Cell

    (1991)
  • S. Yonemura et al.

    J. Cell. Biol.

    (1998)
  • S. Tsukita et al.

    J. Cell. Biol.

    (1994)
  • K.L. Gould et al.

    EMBO J.

    (1989)
  • F. Fazioli et al.

    Oncogene

    (1993)
  • M. Hirao et al.

    J. Cell. Biol.

    (1996)
  • C. Underhill

    J. Cell Sci.

    (1992)
  • K.L. Gould et al.

    J. Cell. Biol.

    (1986)
  • Cited by (136)

    • CircARHGAP12 promotes nasopharyngeal carcinoma migration and invasion via ezrin-mediated cytoskeletal remodeling

      2021, Cancer Letters
      Citation Excerpt :

      Cell movement and migration require dynamic cytoskeletal remodeling, including the dynamic assembly of microtubules, microfilaments, and intermediate filaments, as well as assistance from cytoskeleton-related proteins to change the 3D structure and morphology of cells and drive movement. Cytoskeletal remodeling also plays important roles in cell division, intracellular transport, and maintaining cell shape [38–40]. EZR is a member of the ezrin/radixin/moesin protein family that acts as a linker between the plasma membrane and actin.

    • Ezrin protein overexpression predicts the poor prognosis of pancreatic ductal adenocarcinomas

      2015, Experimental and Molecular Pathology
      Citation Excerpt :

      Ezrin is the best characterized member in the ERM family; it links to aggressive tumor behavior through the involvement in the metastatic process, such as cell adhesion, cell survival, cell motility, and signal transduction, all of which are important for tumor development and progression (Bruce et al., 2007; Guo et al., 2008; Tudor et al., 2014). Accumulating evidences suggest that ezrin is deregulated in borderline lesions and overexpression of ezrin associated with malignancy and metastatic potential of tumors (Yeh et al., 2005; Kitamura et al., 2000; Akisawa et al., 1999), and also indicated that malignant tumors with ezrin overexpression were more invasive with poor-differentiation, rapid progress, early metastasis and poor prognosis (Mallikarjuna et al., 2007; Hara et al., 2008). Recently, researchers have found that ezrin was overexpressed in pancreatic cancer and had significant correlation with its malignance (Clucas et al., 2014; Capello et al., 2013; Kawahara et al., 2013).

    View all citing articles on Scopus
    1

    To whom correspondence should be addressed. Fax: (402) 559-4651.

    View full text