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The Cysteine- and Glycine-Rich LIM Domain Protein CRP2 Specifically Interacts with a Novel Human Protein (CRP2BP),☆☆

https://doi.org/10.1006/bbrc.2000.3187Get rights and content

Abstract

We used the interaction trap to isolate a novel human protein that specifically interacts with the double LIM domain protein CRP2. This protein, designated CRP2BP (for CRP2 binding partner), was previously postulated by sequencing contigs of human chromosome 20. The observed interaction is mediated via the LIM1 domain of CRP2 and is of functional relevance in cellular environment. This novel single copy gene spans approximately 45-bp and is organized into at least ten exons. CRP2BP is expressed in all human tissues tested, with a major mRNA of 4-kb in size and an additional 3.2-kb transcript in placenta.

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    The nucleotide sequence for the CRP2BP cDNA sequence is stored in the Genbank under Accession Number AF252257.

    ☆☆

    Abbreviations used: CRP, cysteine- and glycine-rich protein; CSRP, gene encoding CRP protein; LIM, specific double zinc finger motif; LIM1, amino-terminal LIM domain of CRP; LIM2, carboxyl-terminal LIM domain of CRP protein; MLP, muscle LIM domain protein; PCR, polymerase chain reaction; SD, synthetic dropout medium.

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