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Role of PKC and TGF-β Receptor in Glucose-Induced Proliferation of Smooth Muscle Cells

https://doi.org/10.1006/bbrc.2001.4310Get rights and content

Abstract

The role of protein kinase C (PKC) and transforming growth factor (TGF)-β in the proliferation of vascular smooth muscle cells (SMCs) under a high glucose condition was investigated. [3H]-thymidine incorporation under 20 mM glucose was significantly accelerated compared with that under 5.5 mM glucose, and this increase was inhibited by an anti-TGF-β antibody or a PKC-β specific inhibitor, LY333531. The amount of active and total TGF-β1 in the conditioned media did not differ between 5.5 and 20 mM glucose. However, the expression of TGF-β receptor type II under 20 mM glucose was significantly increased, but that of the TGF-β receptor type I was not. This increased expression of the TGF-β receptor type II was prevented by LY333531. These observations suggest that the increased expression of the TGF-β receptor type II via PKC-β plays an important role in the accelerated proliferation of SMCs under a high glucose condition, leading to the development of diabetic macroangiopathy.

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