Elsevier

Cellular Immunology

Volume 214, Issue 2, 15 December 2001, Pages 110-122
Cellular Immunology

Regular Article
Observation of Antigen-Dependent CD8+ T-Cell/ Dendritic Cell Interactions in Vivo

https://doi.org/10.1006/cimm.2001.1895Get rights and content

Abstract

In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naı̈ve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.

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    1

    To whom correspondence and reprint requests should be addressed. Fax: (303) 398-1396. E-mail: [email protected].

    2

    Present address: 3M Corporation, St. Paul, MN 55144-1000.

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