Elsevier

Developmental Biology

Volume 177, Issue 1, 10 July 1996, Pages 15-29
Developmental Biology

Regular Article
Clustering and Functional Cooperation of Ng-CAM and Axonin-1 in the Substratum-Contact Area of Growth Cones

https://doi.org/10.1006/dbio.1996.0141Get rights and content
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Abstract

Growth cones and neurites of chicken dorsal root ganglia neurons cultured on laminin, Ng-CAM, or axonin-1 exhibit substratum-dependent morphology and growth patterns which are accompanied by distinctive distributions of axonin-1 and Ng-CAM in the growth cone membrane. On either Ng-CAM or axonin-1 substratum, both Ng-CAM and axonin-1 were depleted from some areas of the apical growth cone membrane. In contrast, on laminin, both axonin-1 and Ng-CAM remained randomly distributed. Removal of axonin-1 from growth cones resulted in a blockage of neurite outgrowth on both Ng-CAM and axonin-1 substrata, indicating that in these neurons axonin-1 cooperates with Ng-CAM in the activation of axon growth. Based on these results possible molecular models for cooperation between axonin-1 and Ng-CAM on the growth cone are discussed.

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1

Current address: Department of Neurosciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4975. Fax: (216) 368-4650.