Regular ArticleExpression of β1, β3, β4, and β5 Integrins by Human Lung Carcinoma Cells of Different Histotypes
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Targeted phage display-based pulmonary vaccination in mice and non-human primates
2021, MedCitation Excerpt :Next, the interaction with endogenous α3β1 integrins expressed on the surface of A549 cells were evaluated. The presence of α3β1 integrins on the A549 cell surface has been extensively reported22,23 and it was confirmed by immunofluorescence (Figure 3C). To begin, we applied the biopanning and rapid analysis of selective interactive ligands (BRASIL) methodology24 to demonstrate the binding of CAKSMGDIVC-displaying phage particles to A549 cells, with no detectable binding above background observed for control insertless phage particles (Figure 3D).
Preclinical evaluation of a dual sstr2 and integrin α<inf>v</inf>β<inf>3</inf>-targeted heterodimer [<sup>68</sup>Ga]-NOTA-3PEG<inf>4</inf>-TATE-RGD
2019, Bioorganic and Medicinal ChemistryCitation Excerpt :Both sstr and integrin protein are frequently expressed on the lung carcinomas cell membrane. IHC analysis revealed positive sst2 expression in H69 cells31 and positive integrin αvβ3 expression in A549 cells,32,33 which are in accordance to the PET imaging results. Western blot experiments further confirmed the specific expression of the sstr2 in H69 and integrin αvβ3 in A549 cells (Fig. 6E).
The N-terminal domain of the R28 protein promotes emm28 group A Streptococcus adhesion to host cells via direct binding to three integrins
2018, Journal of Biological ChemistryCitation Excerpt :We have shown that, indeed, it also promotes adhesion to endometrial epithelial cells and decidual cells but also to pulmonary epithelial cells and to keratinocytes, natural targets for GAS invasive infections (Fig. 6). HaCaT and A549 both express α3, α6, β4, and β1 at their surface (54, 55) and that R28Nt interacts with the different corresponding dimers probably accounts for the increase of binding of R28Nt beads to these cell lines (Fig. 6). TC7 cells also express these integrins, but their localization is mainly basolateral (35); this could explain the lower binding of R28Nt to the apical side of TC7 cells compared with the other cell lines.
Radiolabeled Cyclic RGD Peptide Bioconjugates as Radiotracers Targeting Multiple Integrins
2015, Bioconjugate Chemistry