Regular ArticleComparison of Cellular and Nuclear Flow Cytometric Techniques for Discriminating Apoptotic Subpopulations
References (0)
Cited by (128)
A novel photosensitizer DTPP-mediated photodynamic therapy induces oxidative stress and apoptosis through mitochondrial pathways in LA795 cells
2024, Photodiagnosis and Photodynamic TherapyPhytochemical analysis and in vitro cytostatic potential of ethnopharmacological important medicinal plants
2020, Toxicology ReportsCitation Excerpt :After treatment with different plant extracts for 24 h, DL cells were centrifuged (1000xg) and washed twice with chilled PBS, and the cells were resuspended in PBS, fixed with 70 % ethanol at 4 °C. To the cell suspension, 10 μl/mL of 10 mg/mL RNase A solution (DNase free, Sigma-Aldrich) was added anf further stained by propidium iodide solution (50 μl/mL, stock: 1 mg/mL) [18] in dark chamber. The cell was incubated at 37 °C for 20 min and the fluorescence from 20,000 events was determined by a BD FACS Calibur machine (Model FACSC420).
Synergistic effect of piperine and paclitaxel on cell fate via cyt-c, Bax/Bcl-2-caspase-3 pathway in ovarian adenocarcinomas SKOV-3 cells
2016, European Journal of PharmacologyCitation Excerpt :Cells were grown in 6 well plates and after 70% confluency exposed with PIP and PTX alone and in combination. Treated cells were stained with Hoechst 33258 (5 μg/ml) for 30 min at 4 °C in the dark and then imaged under a fluorescent microscope (Elstein and Zucker, 1994). The qualitative analysis of live and apoptotic cells was done using ethidium bromide (EB)/acridine orange (AO) morphology assay (Ribble et al., 2005).
Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells
2015, GeneCitation Excerpt :The cell numbers in the S phase were concomitantly decreased (Fig. 6B). As shown in Fig. 6C, depletion of SCIN also led to an excess accumulation of A549 cells in the sub-G1 phase, which represents apoptotic cells (Elstein and Zucker, 1994; Riccardi and Nicoletti, 2006). Knockdown of SCIN in A549 cells induced cell cycle arrest in the G0/G1 phase and G2/M phase, as well as apoptosis.
Early exercise training improves ischemic outcome in rats by cerebral hemodynamics
2013, Brain ResearchCitation Excerpt :Data were analyzed with FlowJo analysis software. Cell apoptosis was evaluated by nuclear morphology with Hoechst 33258/PI (Beyotime, China) staining (Elstein and Zucker, 1994). Cells stained with Hoechst 33258 (5 μg/ml) and PI (5 μg/ml) for 30 min at 4 °C in the dark were imaged under a fluorescent microscope.