Regular ArticleSpecies-Specific and Conserved Epitopes on Mouse and Human E-Selectin Important for Leukocyte Adhesion
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2009, BloodCitation Excerpt :Rat RB40.34 mAb43 (anti–P-selectin; BD Biosciences PharMingen; 50 μg), 40 μg rat YN1/1.7.4 mAb44 (anti–ICAM-1; Biozol), and 100 μg rat M/K-2 mAb44 (anti–VCAM-1; Chemicon) or 100 μg rat IgG1 isotype control (BD Biosciences PharMingen), and 40 μg rat IgG2b isotype control (Biozol) were injected intravenously 1.5 to 2.0 hours after ear challenge. Rat UZ445 mAb (anti–E-Selectin; kindly provided by R.H.; 200 μg) or 100 μg rat IgM isotype control (BD Biosciences PharMingen) were injected intraperitoneally 1.0 to 1.5 hours after ear challenge. Leukocyte rolling and adhesion dynamics during DTHR were monitored in vivo by video fluorescence microscopy.
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2004, BloodCitation Excerpt :Affinity-purified polyclonal rabbit antibodies against mouse endothelial cell-selective adhesion molecule (ESAM) were generated as described previously.35 The following monoclonal antibodies were purified from hybridoma cells: anti–mouse intercellular adhesion molecule-1 (ICAM-1, YN1/1.7, rat IgG-2b, hybridoma obtained from American Type Culture Collection [Manassas, VA])36; anti–mouse vascular endothelial (VE)–cadherin (11D4.1, rat IgG-2a)37; anti–mouse E-selectin (UZ4, rat IgM),38 anti–mouse P-selectin (RB40.34, rat IgG1),39 and anti–mouse β1-integrin chain (cross-reactive with hamster antigen) (9EG7).40 The following commercially available antibodies were used: fluorescein isothiocyanate (FITC)–labeled antibodies to mouse CD19, CD3ϵ, CD4, CD8, Gr1, and CD11c (BD Pharmingen, San Diego, CA), and anti-7/4 antigen (Serotec, Raleigh, NC).
CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress
2003, BloodCitation Excerpt :Recombinant mouse CCL20 (R&D Systems) was used as a standard. An adhesion assay with lymphocytes on bEnd.3 monolayers was performed under shear stress as described.11,15 Splenoncytes were incubated with or without Ptx (100 ng/mL; List Laboratories) in RPMI (10% fetal calf serum [FCS]; PAN Biotech, Aidenbach, Germany), harvested, and washed once.
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