Elsevier

Experimental Cell Research

Volume 269, Issue 2, 1 October 2001, Pages 266-274
Experimental Cell Research

Regular Article
Species-Specific and Conserved Epitopes on Mouse and Human E-Selectin Important for Leukocyte Adhesion

https://doi.org/10.1006/excr.2001.5317Get rights and content

Abstract

Selectins are C-type, cell surface lectins that are key players in leukocyte adhesion to the blood vessel wall endothelium. We describe here epitopes for a series of novel monoclonal antibodies (moAbs), UZ4-UZ7, directed against mouse E-selectin. All four antibodies specifically bind to mouse E-selectin, but not to P- or L-selectin, and all inhibit the adhesion of granulocytes, peripheral blood lymphocytes, and promyelocytic HL-60 cells to cytokine-activated mouse endothelium. Three moAbs, UZ5, UZ7, and UZ6, specifically inhibit mouse E-selectin-mediated adhesion by binding to epitopes in domains CR1 or CR2. moAb UZ4 inhibits leukocyte adhesion to both human and murine endothelium activated with IL-1 or other proinflammatory stimuli. UZ4 is the first described moAb that detects an epitope in the lectin domain which is conserved in both murine and human E-selectin (CXKKKL), but is not present in the other members of the selectin family, P- and L-selectin. Interestingly, UZ5, UZ6, and UZ7 more efficiently interfere with lymphocyte than with granulocyte adhesion to cytokine-activated endothelium, while UZ4 completely blocks adhesion of PMN, lymphocytes, and HL-60 and U937 cell lines. The data suggest that E-selectin–ligand engagement differs between lymphocytes and PMN, and that these differences may be accentuated by the CR1 and CR2 domains in the E-selectin cell adhesion molecule.

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    To whom correspondence and reprint requests should be addressed at Lehrstuhl für Experimentelle Medizin I, Nikolaus-Fiebiger-Zentrum, Universität Erlangen-Nürnberg, Glueckstr.6, D-91054 Erlangen, Germany. Fax: ++49-9131-8526341. E-mail: [email protected].

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