Elsevier

Genomics

Volume 30, Issue 2, November 1995, Pages 380-384
Genomics

SHORT COMMUNICATION
Molecular Cloning and Chromosomal Assignment of the Human Brain-Type Phosphodiesterase I/Nucleotide Pyrophosphatase Gene (PDNP2)

https://doi.org/10.1006/geno.1995.0036Get rights and content

Abstract

Phosphodiesterase I/nucleotide pyrophosphatase is a widely expressed membrane-bound enzyme that cleaves diester bonds of a variety of substrates. We have cloned brain-type cDNA for this enzyme from rat brain and designated it PD-Iα (M. Narita, J. Goji, H. Nakamura, and K. Sano, 1994,J. Biol. Chem.269: 28235–28242). In this study we have isolated cDNA and genomic DNA encoding human PD-Iα. Human PD-Iα cDNA, designatedPDNP2in HGMW nomenclature, has a 2589-nucleotide open reading frame encoding a polypeptide of 863 amino acids with a calculatedMrof 99,034. Northern blot analysis revealed that human PD-Iα transcript was present in brain, lung, placenta, and kidney. The database analysis showed that human PD-Iα was identical with human autotaxin (ATX), a novel tumor motility-stimulating factor, except that human PD-Iα lacks 156 nucleotides and 52 amino acids of human ATX. Human PD-Iα and human ATX are likely to be alternative splicing products from the same gene. The 5′ region of the humanPDNP2gene contains four putative binding sites of transcription factor Sp1 without typical TATA or CAAT boxes, and there is a potential octamer binding motif in intron 2. From the results of fluorescencein situhybridization, the humanPDNP2gene is located at chromosome 8q24.1.

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