Elsevier

Genomics

Volume 37, Issue 2, 15 October 1996, Pages 147-160
Genomics

Regular Article
Sequence Analysis in the Olfactory Receptor Gene Cluster on Human Chromosome 17: Recombinatorial Events Affecting Receptor Diversity

https://doi.org/10.1006/geno.1996.0536Get rights and content

Abstract

A cosmid clone covering a region of high olfactory receptor (OR) gene density inside the OR gene cluster on human chromosome 17 (17p13.3) was subjected to shotgun automated DNA sequencing. The resulting 40-kb sequence revealed three known OR coding regions, as well as a new OR pseudogene (OR17-25), fused to one of the previously identified OR genes (OR17-24). The suggested mechanism for the generation of this doublet structure involves an initial duplication mediated by flanking repeats and a subsequent deletion via nonhomologous recombination. Sequence analysis further suggests that the two other OR genes present in the cosmid (OR17-40 and OR17-228) may have evolved by ancient tandem duplication of an 11-kb fragment, mediated by recombination between mammalian-wide interspersed repeats. The duplicated genes appear to be complete and potentially functional. Their conserved structure reveals a long upstream intron and a previously uncharacterized 5′ noncoding exon. No additional genes could be discerned in the cosmid, suggesting that the cluster may be part of a dedicated OR subgenome.

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Cited by (76)

  • The human olfactory receptor 17-40: Requisites for fitting into the binding pocket

    2011, Computational Biology and Chemistry
    Citation Excerpt :

    Results reported by Singer (2000) for OR-I7 and Hatt et al. (1999) for OR 17-40 were successfully reproduced by Levasseur et al. (2003), who expressed both rat OR-I7 and human OR 17-40 in mammalian cells to investigate their ligand-binding specificities. The cells expressing recombinant OR-I7 exclusively responded to aldehydes, namely heptanal, octanal and nonanal, in line with previous reports (Araneda et al., 2000; Glusman et al., 1996), where octanal was the main ligand for the rat OR-I7. These results and those of Levasseur et al. (2003) are in fact complementary.

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Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession No. U58675.

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