Elsevier

Genomics

Volume 37, Issue 2, 15 October 1996, Pages 229-233
Genomics

Short Communication
Cloning of Human Lymphocyte-Specific Interferon Regulatory Factor (hLSIRF/hIRF4) and Mapping of the Gene to 6p23–p25

https://doi.org/10.1006/geno.1996.0547Get rights and content

Abstract

The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of ∼5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon–intron structure and a conserved PU.1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23–p25.

References (0)

Cited by (71)

  • Prognostic significance of interferon regulating factor 4 in esophageal squamous cell carcinoma

    2018, Biochemical and Biophysical Research Communications
    Citation Excerpt :

    IRF4 is constitutively expressed in B-cells, and supports B-cell development. In particular, the highest level of IRF4 is observed in plasma cells [28]. In order to further understand the role of tumor-infiltrating B-cells, and the involvement of the humoral immune cells in anti-tumor immunity in ESCC, we sought in this project to examine the prognostic impact of the transcription factor IRF4 in ESCC.

  • Value of melanocytic-associated immunohistochemical markers in the diagnosis of malignant melanoma: A review and update

    2014, Human Pathology
    Citation Excerpt :

    The MUM1 protein comprises 451 amino acids, has a molecular weight of ~52 kd, and is encoded by a gene located on chromosome 6p25.3. In normal tissues, MUM1 is found to be expressed only in lymphoid cells including plasma cells, a subset of germinal center B cells, and activated T cells and in melanocytes [147-149]. Among tumors, MUM1 expression has been reported in a wide spectrum of hematolymphoid neoplasms and melanocytic tumors [149].

  • XA polymorphism in IRF4 affects human pigmentation through a tyrosinase-dependent MITF/TFAP2A pathway

    2013, Cell
    Citation Excerpt :

    A few reports have linked IRF4 to pigment cells. IRF4 is expressed in melanocytes in the skin and in the G361 melanoma cell line (Grossman et al., 1996) as well as in most melanomas (Sundram et al., 2003). Importantly, IRF4 is associated with human pigmentation (Sulem et al., 2007; Han et al., 2008), and IRF4 expression correlates with MITF expression in melanoma cells (Hoek et al., 2008).

  • Colorful DNA polymorphisms in humans

    2013, Seminars in Cell and Developmental Biology
    Citation Excerpt :

    The derived 374F allele is almost fixed in people of European descent, highly frequent in Uygurs from Urumqi (west China), and quite rare in the other populations examined [141,144]. IRF4 (6p25-p23) encodes the interferon regulatory factor 4, detected in malignant melanoma and normal melanocytes [145], is lymphocyte specific in expression and negatively regulates Toll-like-receptor signaling that is central to the activation of innate and adaptive immune systems [146]. An intergenic SNP close to IRF4 was initially found to be associated with freckling in a GWAS on human pigmentation [17].

  • Immunohistology of Non-Hodgkin Lymphoma

    2011, Diagnostic Immunohistochemistry
View all citing articles on Scopus

Sequence data from this article have been deposited with EMBL/GenBank Data Libraries under Accession Nos. U52682 and U52683 for the cDNA and promoter sequence, respectively. The gene symbol IRF4 has been adopted for this locus.

1

These authors contributed equally to this work.

2

To whom correspondence should be addressed at the Amgen Institute, Ontario Cancer Institute, 620 University Avenue, M5G 2C1, Toronto, Ontario, Canada. Telephone: (416) 204-2236. Fax: (416) 204-5300.

View full text