Elsevier

Genomics

Volume 41, Issue 3, 1 May 1997, Pages 485-488
Genomics

SHORT COMMUNICATION
Aphidicolin-Induced FRA3B Breakpoints Cluster in Two Distinct Regions

https://doi.org/10.1006/geno.1997.4690Get rights and content

Abstract

The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin induction of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with breakpoints in the 3p14.2 region. These breakpoints were localized to two distinct clusters, separated by 200 kb, that lie on either side of a region of frequent breakage within FRA3B as observed by FISH analysis. Seven proximal aphidicolin-induced breakpoints were localized at or near the end of a THE element. The THE-1 element is flanked by LINE andAlurepetitive elements. The eight distal aphidicolin-induced breakpoints clustered in a region capable of forming multiple hairpin-like structures. Thus repetitive elements and hairpin-like structures may be responsible for chromosome fragility in this region.

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To whom correspondence and reprint requests should be addressed at Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, 800C Hilton, The Mayo Clinic, 200 First Street S.W., Rochester, MN 55905. Telephone: (507) 266-0309. Fax: (507) 266-5193. E-mail: [email protected].

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