Elsevier

Genomics

Volume 44, Issue 1, 15 August 1997, Pages 83-93
Genomics

Regular Article
Cloning, Structural Analysis, and Chromosomal Localization of the HumanCSRP2Gene Encoding the LIM Domain Protein CRP2,☆☆

https://doi.org/10.1006/geno.1997.4855Get rights and content

Abstract

TheCSRP2gene encoding the LIM domain protein CRP2 was originally identified in quail based on its strong transcriptional suppression in transformed avian fibroblasts. Here we have isolated a humanCSRP2cDNA clone encoding a 193-amino-acid human CRP2 (hCRP2) protein with 96.4% amino acid sequence identity to the avian homolog. TheCSRP2cDNA clone was used to isolateCSRP2-related clones from λEMBL3 and P1 libraries of human genomic DNA. The complete organization of theCSRP2gene was determined by nucleic acid hybridization, transcriptional mapping, and nucleotide sequence analysis. The gene spans a total of approximately 22 kb and contains six exons. The coding region is confined to exons 2–6 and predicts a hCRP2 protein identical in its amino acid sequence to the protein deduced from theCSRP2cDNA clone. By fluorescencein situhybridization using both λEMBL3 and P1 library clones as hybridization probes and a new method for computerized signal localization,CSRP2was mapped to chromosome subband 12q21.1, a region frequently affected by deletion or breakage events in various tumor types. The library screens also led to the isolation of aCSRP2-related pseudogene,CSRP2P,which carried several extensive deletions and nucleotide substitutions but no intervening sequences in comparison to theCSRP2cDNA sequence. By physical linkage and fluorescencein situhybridization,CSRP2Pwas mapped to chromosome subband 3q21.1.

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      Proteins containing LIM (Lin-11, isl-1, and mec-3) domains play an important role in the cell proliferation and differentiation in many cell types (Dawid et al., 1998; Bach, 2000; Kadrmas and Beckerle, 2004). As a subgroup of the LIM family, the cysteine-rich protein (CRP) family, including CRP1, CRP2, and CRP3 has been identified in various species, such as reptiles, fishes, birds, and mammals (Fung et al., 1995; Weiskirchen et al., 1995; Weiskirchen et al., 1997; Delalande and Rescan, 1998; Henderson et al., 2002). The three CRP family members shared two evolutionarily conserved LIM zinc-finger domains and are essential for muscle cell development and differentiation (Louis et al., 1997; Pomies et al., 1997; Chang et al., 2003; Duan et al., 2003).

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    Sequence data from this article have been deposited with the GenBank Data Library under Accession Nos. U57646, U72535, U95017, and U95018.

    ☆☆

    D. E. RooneyB. H. Czepulkowski, Eds

    1

    To whom correspondence should be addressed at the Institute of Biochemistry, University of Innsbruck, Peter-Mayr-Str. 1a, A-6020 Innsbruck, Austria. Telephone: +43-512-507-5271. Fax: +43-512-507-2894. E-mail: [email protected].

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