Elsevier

Genomics

Volume 46, Issue 1, 15 November 1997, Pages 93-102
Genomics

Regular Article
Genomic Structure and Chromosomal Mapping of the Nuclear Orphan Receptor RORγ (RORC) Gene

https://doi.org/10.1006/geno.1997.4980Get rights and content

Abstract

The nuclear orphan receptor subfamily ROR/RZR is part of the steroid and thyroid hormone/retinoid receptor superfamily and consists of three different genes, α, β, and γ. In this study, we determined the genomic structure of mouse RORγ and the chromosomal localization of both mouse RORγ and human RORγ (HGMW-approved symbol RORC). The genomic structure of the mouse RORγ gene was derived from the analysis of P1 vector clones containing large genomic fragments encoding RORγ. These results revealed that the mRORγ gene has a complex structure consisting of 11 exons separated by 10 introns spanning more than 21 kb of genomic DNA. The DNA-binding domain is contained in two exons, 3 and 4, each encoding one zinc-finger. The splice site between exon 3 and exon 4 is identical to that found in RAR and TR3 receptors. RORγ is expressed as two mRNAs, 2.3 and 3.0 kb in size, that are derived by the use of alternative polyadenylation signals. We show by fluorescencein situhybridization that the mouse RORγ gene is located on chromosome 3, in a region that corresponds to band 3F2.1–2.2. The human RORγ was mapped to chromosome region 1q21. The results demonstrate that the RORγ genes are located in chromosomal regions that are syntenic between mouse and human.

References (44)

  • T. Stokke et al.

    A physical map of chromosome 20 established using fluorescencein situ

    Genomics

    (1995)
  • M. Tini et al.

    Functional interactions between retinoic acid receptor-related orphan receptor (ROR alpha) and the retinoic acid receptors in the regulation of the gamma F-crystallin promoter

    J. Biol. Chem.

    (1995)
  • P. Tontonoz et al.

    Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor

    Cell

    (1994)
  • J. Whang-Peng et al.

    Sequential analysis of 43 patients with non-Hodgkins's lymphoma: Clinical correlations with cytogenetic, histologic, immunophenotyping and molecular studies

    Blood

    (1995)
  • M. Wickens

    How the messenger got its tail: Addition of poly(A) in the nucleus

    Trends Bio.

    (1990)
  • H. Adachi et al.

    Suppression by retinoids of the induction of the CAAT/enhancer-binding protein α and the nuclear receptors PPARγ and RORγ during adipocyte differentiation of 3T3-L1 cells

    Mol. Cell. Differ.

    (1996)
  • S. Austin et al.

    Induction of the nuclear orphan receptor RORγ during adipocyte differentiation of D1 and 3T3-L1 cells. Cross-talk between nuclear receptor pathways

    Cell Growth Differ.

    (1997)
  • C. Carlberg et al.

    RZRs, a new family of retinoid-related orphan receptors that function as both monomers and homodimers

    Mol. Endocrinol.

    (1994)
  • C. Chomienne et al.

    The retinoic acid receptor α gene is rearranged in retinoic acid-sensitive promyelocytic leukemias

    Leukemia

    (1990)
  • L.M. De Luca

    Retinoids and their receptors in differentiation, embryogenesis and neoplasia

    FASEB J.

    (1991)
  • J. Devereux et al.

    A comprehensive set of sequence analysis programs for the VAX

    Nucleic Acids Res.

    (1984)
  • Cited by (0)

    Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. AF019655–AF019660.

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