Elsevier

Genomics

Volume 72, Issue 2, 1 March 2001, Pages 169-179
Genomics

Regular Article
Physical and Transcriptional Map of a 311-kb Segment of Chromosome 18q21, a Candidate Lung Tumor Suppressor Locus

https://doi.org/10.1006/geno.2000.6454Get rights and content

Abstract

Here, we report the complete genomic sequence and the characterization of the 311-kb region of 18q21, a candidate tumor suppressor locus containing a region of homozygous deletion in a lung cancer cell line, Ma29. This region contained two known genes, SMAD4 and ME2 (mitochondrial malate oxydoreductase), and two novel genes, D29 (deleted in Ma29 HGMW-approved symbol ELAC1), encoding an evolutionarily conserved protein, and B29 (beside the Ma29 deletion HGMW-approved symbol C18orf3), with no significant homology to any known genes. The deleted DNA segment in Ma29, which was estimated to be 195 kb in size, included all the coding exons of ME2 and D29, but not the coding exons of SMAD4 and B29. The deleted region also included exon 0, a 5′-noncoding exon, of SMAD4, and the expression of SMAD4 was greatly reduced in Ma29 cells. Mutations of SMAD4 and D29 were detected in 1 of 45 lung cancer cell lines examined, while those of ME2 and B29 were not detected, indicating that these four genes are not major targets for 18q21 deletions. The physical and transcriptional map constructed in this study will provide basic information for the identification of a tumor suppressor gene(s) at 18q21 involved in lung carcinogenesis.

References (40)

  • E.R. Fearon et al.

    Identification of a chromosome 18q gene that is altered in colorectal cancers

    Science

    (1990)
  • K.M. Fong et al.

    Tumor progression and loss of heterozygosity at 5q and 18q in non-small cell lung cancer

    Cancer Res.

    (1995)
  • S.A. Hahn et al.

    DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1

    Science

    (1996)
  • S.A. Hahn et al.

    Homozygous deletion map at 18q21.1 in pancreatic cancer

    Cancer Res.

    (1996)
  • K. Hamada et al.

    Two regions of homozygous deletion clusters at chromosome band 9p21 in human lung cancer

    Genes Chromosomes Cancer

    (2000)
  • W. Hilgers et al.

    Novel homozygous deletions of chromosomal band 18q22 in pancreatic adenocarcinoma identified by STS marker scanning

    Genes Chromosomes Cancer

    (1999)
  • W. Hilgers et al.

    Homozygous deletions inactivate DCC, but not MADH4/DPC4/SMAD4, in a subset of pancreatic and biliary cancers

    Genes Chromosomes Cancer

    (2000)
  • K. Huebner et al.

    The role of deletions at the FRA3B/FHIT locus in carcinogenesis

    Rec. Results Cancer Res.

    (1998)
  • T. Inoue et al.

    Loss of heterozygosity on chromosome 18q in cohesive-type gastric cancer is associated with tumor progression and poor prognosis

    Clin. Cancer Res.

    (1998)
  • Cited by (19)

    • Genes for tRNA recycling are upregulated in response to infection with Theiler's mouse encephalitis virus

      2022, Biochemical and Biophysical Research Communications
      Citation Excerpt :

      On the other hand, tRNase ZS has very limited pre-tRNA-cleaving activity compared with tRNase ZL [6,7]. This property together with the fact that the tRNase ZS gene is dispensable for viability of at least one human cell line made us curious about its physiological role [8]. Another tRNA maturation enzyme, TRNT1 (or the CCA-adding enzyme), catalyzes the addition of CCA nucleotides [9].

    • Structure of primate and rodent orthologs of the prostate cancer susceptibility gene ELAC2

      2004, Biochimica et Biophysica Acta - Gene Structure and Expression
    View all citing articles on Scopus

    Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under Accession Nos. AP001169, AB043547, AB045122, AB029151, and AB042647.

    1

    To whom correspondence should be addressed. Telephone: 81-3-3547-5272. Fax: 81-3-3542-0807. E-mail: [email protected].

    View full text