Elsevier

Genomics

Volume 79, Issue 4, April 2002, Pages 598-602
Genomics

Regular Article
Correct Identification of Genes from Serial Analysis of Gene Expression Tag Sequences

https://doi.org/10.1006/geno.2002.6730Get rights and content

Abstract

SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3′ EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method.

References (23)

  • A. Lal

    A public database for gene expression in human cancers

    Cancer Res.

    (1999)
  • Cited by (0)

    *

    These authors contributed equally to this project.

    To whom correspondence and reprint requests should be addressed. Fax: (773) 702-3002. E-mail: [email protected].

    View full text