Elsevier

Genomics

Volume 80, Issue 1, July 2002, Pages 13-20
Genomics

Regular Article
A Genome Scan for Loci Associated with Aerobic Running Capacity in Rats

https://doi.org/10.1006/geno.2002.6797Get rights and content

Abstract

Aerobic capacity is a complex trait that defines the efficiency to use atmospheric oxygen as an electron acceptor in energy transfer. Copenhagen (COP) and DA inbred rat strains show a wide difference in a test for aerobic treadmill running and serve as contrasting genetic models for aerobic capacity. A genome scan was carried out on an F2(COP×DA) segregating population (n=224) to detect quantitative trait loci (QTLs) associated with aerobic running capacity. Linkage analysis revealed a significant QTL on chromosome 16 (lod score, 4.0). A suggestive linkage was found near the p-terminus of chromosome 3 (lod score, 2.2) with evidence of an interaction with another QTL on chromosome 16 (lod score, 2.9). All three QTLs showed a dominant mode of inheritance in which the presence of at least one DA allele was associated with a greater distance run. These results represent the first aerobic capacity QTLs identified in genetic models.

References (46)

  • G Erikssen

    Changes in physical fitness and changes in mortality

    Lancet

    (1998)
  • C.H Warden

    Chromosomal localization of lipolytic enzymes in the mouse: pancreatic lipase, colipase, hormone-sensitive lipase, hepatic lipase, and carboxyl ester lipase

    J. Lipid Res.

    (1993)
  • J.E Baldwin et al.

    The evolution of metabolic cycles

    Nature

    (1981)
  • C Bouchard

    Aerobic performance in brothers, dizygotic and monozygotic twins

    Med. Sci. Sports Exerc.

    (1986)
  • V Klissouras

    Heritability of adaptive variation

    J. Appl. Physiol.

    (1971)
  • K Wasserman et al.

    Principles of Exercise Testing and Interpretation: Including Pathophysiology and Clinical Applications

    (1999)
  • M.S Bray

    Genomics, genes, and environmental interaction: the role of exercise

    J. Appl. Physiol.

    (2000)
  • C Bouchard

    Genomic scan for maximal oxygen uptake and its response to training in the HERITAGE family study

    J. Appl. Physiol.

    (2000)
  • J Flint et al.

    Finding the molecular basis of quantitative traits: successes and pitfalls

    Nat. Rev. Genet.

    (2001)
  • J.C Barbato

    Spectrum of aerobic endurance running performance in eleven inbred strains of rats

    J. Appl. Physiol.

    (1998)
  • E.S Lander et al.

    Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results

    Nat. Genet.

    (1995)
  • H.J Jacob

    Functional genomics and rat models

    Genome Res.

    (1999)
  • J.P Rapp

    Genetic analysis of inherited hypertension in the rat

    Physiol. Rev.

    (2000)
  • L.G Koch et al.

    Artificial selection for intrinsic aerobic endurance running capacity in rats

    Physiol. Genomics

    (2001)
  • E.S Lander et al.

    Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps

    Genetics

    (1989)
  • J.W van Ooijen

    Accuracy of mapping quantitative trait loci in autogamous species

    Theor. Appl. Genet.

    (1992)
  • A Darvasi et al.

    A simple method to calculate resolving power and confidence interval of QTL map location

    Behav. Genet.

    (1997)
  • D.A Nickerson

    DNA sequence diversity in a 9.7-kb region of the human lipoprotein lipase gene

    Nat. Genet.

    (1998)
  • C Garenc

    Evidence of LPL gene-exercise interaction for body fat and LPL activity: The HERITAGE Family Study

    J. Appl. Physiol.

    (2001)
  • A Bottger

    Quantitative trait loci influencing cholesterol and phospholipid phenotypes map to chromosomes that contain genes regulating blood pressure in the spontaneously hypertensive rat

    J. Clin. Invest.

    (1996)
  • M Pravenec

    A genetic map of the rat derived from recombinant inbred strains

    Mamm. Genome

    (1996)
  • L.A Campfield et al.

    Strategies and potential molecular targets for obesity treatment

    Science

    (1998)
  • Cited by (31)

    • Cardiovascular research

      2019, The Laboratory Rat
    • Postnatal training of 129/Sv mice confirms the long-term influence of early exercising on the motor properties of mice

      2016, Behavioural Brain Research
      Citation Excerpt :

      Possible chromosomal locations of regulating genes showed quantitative trait loci (QTL) related to open-maze locomotion [3], open-field activity [4], rotarod performance [5], duration and speed of wheel running [6], and endurance training [7]. One of the QTL involved in the exercise endurance has homologues in rats (D16Rat17) [8], and humans (D13S787, D22S274) [7]. In support of the genetic influence on locomotion, muscle phenotype varies greatly amongst mice strains.

    • Repeatability of exercise behaviors in mice

      2009, Physiology and Behavior
      Citation Excerpt :

      Treadmill testing for assessment of endurance/aerobic capacity in rodents has been generally preferred to swimming tests since rodents do not display consistent swimming behaviors (e.g. animals will bob, float, and/or dive) and these behaviors skew any data investigating aerobic capacity [20]. Several variations of exercise treadmill protocols have been used with rodents [4,18,22,24,25,31,36,39]; however, in the current literature, limited studies report a measure of repeatability of forced treadmill testing within animal [6,17,31]. These studies report within animal repeatability of VO2max measurements, using enclosed treadmill protocols ranging from r = 0.42 to 0.97 [6,17,31].

    • Comparative Genomics for Detecting Human Disease Genes

      2008, Advances in Genetics
      Citation Excerpt :

      To date, there have been more than 2900 published QTL studies in the mouse [according to the Mouse Genome Informatics (MGI) database] (Flint et al., 2005) and 536 QTL papers published with over 1000 QTLs reported for different physiological and pathophysiological traits in the rat. These papers include investigations of the genetic basis of blood pressure (Rapp, 2000), diabetes (Galli et al., 1996; Jacob et al., 1992; Pravenec et al., 1996), cardiovascular disease (Moreno et al., 2003; Stoll et al., 2001), stroke (Rubattu et al., 1996), ethanol preference (Murphy et al., 2002), behavioral conditioning and anxiety (Fernandez‐Teruel et al., 2002; Flint, 2003), fat accumulation (Tanomura et al., 2002), arthritis (Olofsson et al., 2003a), copper metabolism (de Wolf et al., 2002), pituitary tumor growth (Wendell and Gorski, 1997), aerobic capacity (Ways et al., 2002), and chemical carcinogenesis (De Miglio et al., 2002). QTL mapping has been applied to many other organisms as well: zebrafish for QTL mapping of phenotypes with clinical implications such as behavior (Wright et al., 2006); cows for mapping of agriculturally important traits such as milk quality and quantity (Grisart et al., 2002; Malek et al., 2006), which may shed light on fat content and nutrition in human milk; hip dysplasia in dogs (Todhunter et al., 2003) with relevance to human arthritis.

    • Heritability of endurance traits from animal research models

      2019, Routledge Handbook of Sport and Exercise Systems Genetics
    View all citing articles on Scopus
    *

    To whom correspondence and reprint requests should be addressed. Fax: (419) 383-6168. E-mail: [email protected].

    View full text