Regular ArticleEstablishment of Human Colonic Epithelial Cells in Long-Term Culture☆
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Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells
2012, Toxicology in VitroCitation Excerpt :After 48 h crypts detached and cells around the crypts were getting in suspension. The epithelial nature of the attached monolayer clusters was proven by the specific epithelial antibody pan-cytokeratin (Deveney et al., 1996; Vidrich et al., 1988). The majority of attached cells reacted positive with the antibody and only some cells showed a positive staining for vimentin indicating the presence of fibroblasts or other mesenchymal cells (Mahida et al., 1997).
Intestinal Epithelial Stem Cells and Progenitors
2006, Methods in EnzymologyCitation Excerpt :Typically, most epithelial cells isolated with EDTA failed to attach and died within a few hours (Quaroni et al., 1979). Similar difficulties with viability of EDTA‐isolated epithelium were experienced by other groups (Deveney et al., 1996; Evans et al., 1992). As mentioned previously, if steps are taken to override isolation‐induced anoikis, chelation‐isolated epithelium is viable and can be cultured.
Reconsidering cell line cross-contamination in NCOL-1 (multiple letters)
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Presented at the Annual Meeting of the Association for Academic Surgery, Dearborn, Michigan, November 8–11, 1995.