Regular Article
Heat Shock Protein 27 Plays Two Distinct Roles in Controlling Human Breast Cancer Cell Migration on Laminin-5

https://doi.org/10.1006/mcbr.1999.0130Get rights and content

Abstract

It has recently been reported that phosphorylation of the small heat shock protein 27 (hsp27) enhances p38 MAP kinase dependent migration of bovine and human vascular endothelial cells. We have examined the role of hsp27 in controlling the constitutive migration of human breast cancer cells on the extracellular matrix molecule laminin-5. In a haptotaxis assay, anisomycin- or heat shock-induced phosphorylation of hsp27 enhances migration of MDA-MB-231 breast cancer cells constitutively overexpressing hsp27. Under these conditions, hsp27 redistributes to the nucleus. Unphosphorylated hsp27, which remains in the cytosol, induces resistance to a subset of drugs that inhibit haptotactic migration of these cells. We conclude that hsp27 plays two distinct roles in controlling migration of breast cancer cells: phosphorylated hsp27 enhances migration, while unphosphorylated hsp27 can sustain migration in the presence of inhibitory drugs.

References (27)

  • Q. Chen et al.

    J. Biol. Chem.

    (1994)
  • P.R.L.A. van den IJssel et al.

    Biochem. Biophys. Res. Commun.

    (1998)
  • D.R. Ciocca et al.

    J. Natl. Cancer Inst.

    (1993)
  • A.P. Arrigo et al.
  • J. Landry et al.

    Biochem. Cell Biol.

    (1995)
  • J. Guay et al.

    J. Cell Sci.

    (1997)
  • J.N. Lavoie et al.

    Mol. Cell Biol.

    (1995)
  • P. Lemieux et al.

    Invasion Metastasis

    (1997)
  • A.E. Eskenazi et al.

    Clin. Exp. Metastasis

    (1998)
  • B. Alberts et al.

    Molecular Biology of the Cell

    (1989)
  • G.E. Plopper et al.

    Breast Cancer Res. Treat.

    (1998)
  • S. Stahl et al.

    J. Cell Sci.

    (1997)
  • G. Giannelli et al.

    Science

    (1997)
  • Cited by (0)

    1

    To whom correspondence should be addressed at Department of Biological Sciences, University of Nevada, Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4004. Fax: (702) 895-2956. E-mail: [email protected].

    View full text