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Presence of the LEE (locus of enterocyte effacement) in pig attaching and effacing Escherichia coli and characterization of eae, espA, espB and espD genes of PEPEC (pig EPEC) strain 1390

https://doi.org/10.1006/mpat.1999.0346Get rights and content

Abstract

In the present study, attaching and effacing Escherichia coli (AEEC) O45 isolates from post-weaning pigs with diarrhoea were examined for the presence of the LEE (locus of enterocyte effacement) using various DNA probes derived from the LEE of human enteropathogenic E. coli (EPEC) strain E2348/69. The LEE fragment was conserved among the eae -positive pig isolates. The attaching and effacing activity of PEPEC (pig EPEC) O45 isolates is highly correlated with the presence of the LEE. Nevertheless, for some PEPEC isolates, the insertion site of the LEE is different or has diverged during evolution. The presence of the LEE fragment in PEPEC isolates provides further evidence that the LEE region is conserved among AEEC of different animal origins. In addition, the nucleotide sequence of the region containing the eae gene and esp genes of a pig AEEC isolate, strain 1390, was determined. Among examined Eae proteins, Eae of strain 1390 showed the highest similarity with Eae belonging to the β intimin group such as the Eae of rabbit AEEC. Moreover, all pig strains that produced attaching and effacing lesions in piglets and pig ileal explants belonged to the β intimin group. The deduced amino acid sequences of the EspA, EspB and EspD proteins of strain 1390 showed particularly strong homology to those of AEEC strains presenting a β intimin allele. Thus, pig AEEC possess the LEE sequences, and for the strain 1390, sequences of theeae and esp regions are related to those of other AEEC, in particular, strains presenting a β intimin allele, such as the rabbit AEEC.

References (46)

  • HW Moon et al.

    Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines

    Infect Immun

    (1983)
  • P Hélie et al.

    Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K “E65”strain

    Infect Immun

    (1991)
  • BH Janke et al.

    Attaching and effacing Escherichia coli infections in calves, pigs, lambs, and dogs

    J Vet Diagn Invest

    (1989)
  • C Zhu et al.

    Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea

    Infect Immun

    (1994)
  • CH Pai et al.

    Experimental infection of infant rabbits with verotoxin-producing Escherichia coli

    Infect Immun

    (1986)
  • A Takeuchi et al.

    Scanning and transmission electron microscopic study of Escherichia coli O15 (RDEC-1) enteric infection in rabbit

    Infect Immun

    (1978)
  • MJ Albert et al.

    Hafnia alvei, a probable cause of diarrhea in humans

    Infect Immun

    (1991)
  • DB Schauer et al.

    Attaching and effacing locus of a Citrobacter freundii biotype that causes transmissible murine colonic hyperplasia

    Infect Immun

    (1993)
  • MS Donnenberg et al.

    A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence

    Mol Microbiol

    (1992)
  • MK Wolf et al.

    Characterization of the plasmid from Escherichia coli RDEC-1 that mediates expression of adhesin AF/R1 and evidence that AF/R1 pili promote but are not essential for enteropathogenic disease

    Infect Immun

    (1988)
  • TK McDaniel et al.

    A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens

    Proc Natl Acad Sci USA

    (1995)
  • KG Jarvis et al.

    Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system

    Infect Immun

    (1996)
  • MS Donnenberg et al.

    A second chromosomal gene necessary for intimate attachment of enteroÍpathogenic Escherichia coli to epithelial cells

    J Bacteriol

    (1993)
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    Author for correspondence. E-mail: [email protected]

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