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Extreme Intraspecific Mitochondrial DNA Sequence Divergence inGalaxias maculatus(Osteichthys: Galaxiidae), One of the World's Most Widespread Freshwater Fish

https://doi.org/10.1006/mpev.1998.0554Get rights and content

Abstract

Biogeographic controversies surrounding the widespread freshwater fish,Galaxias maculatus,were addressed with DNA sequence data. Mitochondrial cytochromeband 16S rRNA sequences were obtained from representatives of six populations of this species. Substantial levels of cytochromeb(maximum 14.6%) and 16S rRNA sequence divergence (maximum 6.0%) were detected between western Pacific (Tasmania–New Zealand) and South American (Chile–Falkland Islands) haplotypes. A considerable level of divergence was also detected between Tasmanian and New Zealand haplotypes (maximum 5.1%) and within and among Chilean and Falkland Island G. maculatus (maximum 3.8%). The phylogenetic structure of haplotypes conflicts with the accepted pattern of continental fragmentation. Molecular clock calibrations suggest that haplotype divergences postdate the fragmentation of Gondwana. These findings point to marine dispersal rather than ancient vicariance as an explanation for the wide distribution. The phylogenetic structure of South American haplotypes was not consistent with their geographic distribution. We consider factors such as population divergence, population size, dispersal, secondary contact, and philopatry as potential causes of the high level of mtDNA nucleotide diversity in this species.

References (47)

  • B.W. Bowen et al.

    Phylogeography of the sardines (Sardinops

    Evolution

    (1997)
  • W.M. Brown et al.

    Mitochondrial DNA sequences of primates: Tempo and mode of evolution

    J. Mol. Evol.

    (1982)
  • R.C. Craw

    Generalised tracks and dispersal in biogeography: A response to R. M. McDowall

    Syst. Zool.

    (1979)
  • L. Croizat et al.

    Centers of origin and related concepts

    Syst. Zool.

    (1974)
  • J.J. Doyle

    Gene trees and species trees: Molecular systematics as one-character taxonomy

    Syst. Bot.

    (1992)
  • J. Felsenstein

    Confidence limits on phylogenies: An approach using the bootstrap

    Evolution

    (1985)
  • Felsenstein, J. 1995, PHYLIP (Phylogeny Inference Package), Department of Genetics, University of Washington,...
  • D.J. Jellyman et al.

    First record of the Australian long-finned eel,Anguilla reinhardtii,

    Mar. Freshw. Res.

    (1996)
  • T.D. Kocher et al.

    Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers

    Proc. Natl. Acad. Sci. USA

    (1989)
  • A.P. Martin et al.

    Body size, metabolic rate, generation time, and the molecular clock

    Proc. Natl. Acad. Sci. USA

    (1993)
  • R.M. McDowall

    Some points of confusion in galaxiid nomenclature

    Copeia

    (1967)
  • R.M. McDowall

    The galaxiid fishes of New Zealand

    Bull. Mus. Comp. Zool. Harv.

    (1970)
  • R.M. McDowall

    The galaxiid fishes of South America

    Zool. J. Linn. Soc.

    (1971)
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