Regular ArticleExtreme Intraspecific Mitochondrial DNA Sequence Divergence inGalaxias maculatus(Osteichthys: Galaxiidae), One of the World's Most Widespread Freshwater Fish
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2013, Molecular Phylogenetics and EvolutionCitation Excerpt :Based on mtDNA CR sequences, strict molecular clock model implemented in the program BEAST v1.6.1 was applied to estimate divergence time. The substitution rate was constrained to the extent of 0.5–0.9% divergence per site per million year for freshwater fish mtDNA genes (Martin and Palumbi, 1993; Taylor et al., 1997; Waters and Burridge, 1999; Zardoya and Doadrio, 1999). Two independent runs were performed with 2 × 107 MCMC iterations.
Phylogenetic relationships and demographic histories of the Atherinidae in the Eastern Atlantic and Mediterranean Sea re-examined by Bayesian inference
2012, Molecular Phylogenetics and EvolutionCitation Excerpt :Sequence divergence data confirms the species status of the punctuated and non-punctuated forms, which show a divergence around 15% for D-loop, 5% for 12s rRNA and 7% for 16s rRNA with the rest of species in the group. Mitochondrial DNA sequence divergences seldom exceed a few percent within fish species, with the highest values reported in literature (4.6–6.0% at 16S rRNA in Galaxias maculatus; Waters and Burridge, 1999) being lower than the values in our study. In regards to the highly differentiated populations of A. boyeri and A. presbyter, the amount of sequence divergence found places them on the limit between species and sub-species.
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