Regular Article
The Evolution of Nuclear Receptors: Evidence from the Coral Acropora

https://doi.org/10.1006/mpev.2001.0994Get rights and content

Abstract

We have amplified and sequenced PCR products derived from 10 nuclear receptor (NR) genes from the anthozoan cnidarian Acropora millepora, including five products corresponding to genes not previously reported from the phylum Cnidaria. cDNAs corresponding to seven of these products were sequenced and at least three encode full-length proteins, increasing the number of complete cnidarian NR coding sequences from one to four. All clear orthologs of Acropora NRs either lack an activation domain or lack a known ligand, consistent with the idea that the ancestral nuclear receptor was without a ligand. Phylogenetic analyses indicate that most, and possibly all, presently identified cnidarian NRs are members of NR subfamily 2, suggesting that the common ancestor of all known nuclear receptors most resembled members of this subfamily.

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Supplementary Files

1. Caption.

Alignment of nuclear receptor amino acid sequences used in phylogenetic analyses.

A. Amino acid alignment of the DNA binding domain (DBD) region. The P box and D box regions are shown above the alignment.

B. Amino acid alignment of alpha helices 3-5 of the ligand binding domains (LBDs). The positions of the helices are indicated above the alignment.

C. Amino acid alignment of alpha helices 7-11 of the LBDs. The positions of the helices are indicated above the alignment. A gap in the

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