Elsevier

Plasmid

Volume 44, Issue 2, September 2000, Pages 183-190
Plasmid

Short Communication
Improved Vectors for Nisin-Controlled Expression in Gram-Positive Bacteria

Communicated by D. LeBlanc
https://doi.org/10.1006/plas.2000.1484Get rights and content

Abstract

A set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the two-component signaling mechanism for activating transcription from PnisA in the presence of nisin, was constructed. To test these vectors, Enterococcus faecalis pCF10 plasmid genes prgX, prgY, and prgZ, which respectively encode cytosolic, integral membrane, and cell surface proteins, were cloned downstream of PnisA. Increased protein expression, in the presence of nisin, was demonstrated by Western blot analysis.

References (23)

  • Z. Eichenbaum et al.

    Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: Comparison of induction level and promoter strength

    Appl. Environ. Microbiol.

    (1998)
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