Regular ArticleProduction of Human Lactoferrin in Transgenic Tobacco Plants☆
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A 90-day subchronic toxicology screen of genetically modified rice Lac-3 and its effects on the gut microbiota in Sprague-Dawley rats
2019, Regulatory Toxicology and PharmacologyCitation Excerpt :At the specie level s, Bacteroides uniformis, Bacteroides vulgatus accounted for a higher proportion in the 70% GM group of the male rats compared with the corresponding non-GM group. Nowadays, recombinant LF has been produced in various plants including tobacco, potato and rice (Nandi et al. 2002; Salmon et al., 1998). Expression level of rhLF in rice is higher than in tobacco and potato, making rice grain is an attractive expression system (Nandi et al. 2002).
Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphA <inf>Te</inf> gene expression
2012, Journal of BiotechnologyCitation Excerpt :Progress has also been made in the area of transforming the cyanophycin synthetase (cphA) gene into tobacco and potato plants (Hühns et al., 2008, 2009; Neumann et al., 2005). Tobacco is known to be a good system for the production of recombinant proteins (Salmon et al., 1998; Sehnke et al., 1994; Wang et al., 2001), as it is easy to transform and shows great possibilities for scale-up due to the production of thousands of seeds per plant (Balasubramaniam et al., 2003). Cyanophycin accumulation up to 6.8% of dry weight (dw) in tobacco leaves and 7.5% dw in potato tubers have been reported (Hühns et al., 2008, 2009).
A selectively terminable transgenic rice line expressing human lactoferrin
2010, Protein Expression and PurificationRecombinant human lactoferrin: A valuable protein for pharmaceutical products and functional foods
2010, Biotechnology AdvancesCitation Excerpt :Moreover, it is possible to direct the expression of proteins to specific parts of the plant, such as fruit, seeds, leaves and tubers (Lönnerdal, 2002), targeting that may facilitate processing and/or purification. The expression of rhLF has been achieved in tobacco plants using two constructs containing either the native signal peptide from hLF or the signal peptide from sweet potato sporamin fused to hLF encoding cDNA (Salmon et al., 1998). The N-terminal sequence of the mature protein using either construct was identical to that of native hLF and the molecular mass was similar to that of the native protein.
Recovery of nicotine-free proteins from tobacco leaves using phosphate buffer system under controlled conditions
2010, Bioresource TechnologyPlants as bioreactors: Recent developments and emerging opportunities
2009, Biotechnology AdvancesCitation Excerpt :In order to increase the strength of CaMV35S promoter, multiple copies of its enhancer element have been used (Guerineau et al., 1992). The CaMV35S promoter with its duplicated enhancer sequence has been used to optimize the expression level of several recombinant bio-molecules in plants and enhanced expression levels have been achieved (Ashraf et al., 2005; Dong et al., 2005; Hull et al., 2005a; Mishra et al., 2006; Ruggiero et al., 2000; Salmon et al., 1998; Sharma et al., 2008a,b). A hybrid promoter, mac, was constructed by incorporating part of mas promoter and enhancer region of CaMV35S promoter.
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Neiland, J. B.
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