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Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells

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Mouse Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 633))

Abstract

Limited understanding of the cell biology of the breast and breast cancer hampers our ability to develop new therapeutic approaches. Mouse models of mammary gland development and tumourigenesis are key to developing new insights into the biology of both the normal and diseased tissues. Recent advances have enabled the isolation, molecular characterisation and functional analysis of mouse mammary epithelial cell subpopulations from the normal gland, including subpopulations enriched for stem cell behaviour. Application of these techniques both to the normal mammary gland and to tumour models will promote a better understanding of the nature of the different epithelial cell types in the mammary gland, the origins of mammary tumours and the role of tumour stem cells.

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Acknowledgements

Matthew Smalley is currently funded by Breakthrough Breast Cancer and acknowledges NHS funding to the NIHR Biomedical Research Centre. The author would like to thank Howard Kendrick for his assistance in taking photographs and Katherine Sleeman for comments on the manuscript.

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Authors and Affiliations

  1. Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, SW3 6JB, UK

    Matthew J. Smalley

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  1. Matthew J. Smalley
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Editors and Affiliations

  1. Dept. Biology & Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom

    Andrew Ward

  2. Dept. Biology & Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom

    David Tosh

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Smalley, M.J. (2010). Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells. In: Ward, A., Tosh, D. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 633. Humana Press. https://doi.org/10.1007/978-1-59745-019-5_11

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  • DOI: https://doi.org/10.1007/978-1-59745-019-5_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-772-3

  • Online ISBN: 978-1-59745-019-5

  • eBook Packages: Springer Protocols

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