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Differentiation of Adipose Stem Cells

  • Protocol
Book cover Adipose Tissue Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 456))

Summary

The broad definition of a stem cell is a cell that has the ability to self-renew and differentiate into one or more specialized terminally differentiated cell types. It has become evident that stem cells persist in, and can be isolated from, many adult tissues. Adipose tissue has been shown to contain a population of cells that retain a high proliferation capacity in vitro and the ability to undergo extensive differentiation into multiple cell lineages. These cells are referred to as adipose stem cells and are biologically similar, although not identical, to mesenchymal stem cells derived from the bone marrow. Differentiation causes stem cells to adopt the pheno-typic, biochemical, and functional properties of more terminally differentiated cells. This chapter will provide investigators with some background on stem cells derived from adipose tissue and then provide details on adipose stem cell multilineage differentiation along osteogenic, adipogenic, chondrogenic, and neurogenic lineages.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Center for Gene Therapy, Division of Gene Therapy, Tulane National Primate Research Center, Covington, Louisiana

    Bruce A. Bunnell

  2. Divison of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina

    Bradley T. Estes & Farshid Guilak

  3. Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana

    Jeffrey M. Gimble

Authors
  1. Bruce A. Bunnell
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  2. Bradley T. Estes
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  3. Farshid Guilak
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  4. Jeffrey M. Gimble
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Editor information

Editors and Affiliations

  1. The University of Western Ontario, London, Ontario, Canada

    Kaiping Yang

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Bunnell, B.A., Estes, B.T., Guilak, F., Gimble, J.M. (2008). Differentiation of Adipose Stem Cells. In: Yang, K. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 456. Humana Press. https://doi.org/10.1007/978-1-59745-245-8_12

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  • DOI: https://doi.org/10.1007/978-1-59745-245-8_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-916-1

  • Online ISBN: 978-1-59745-245-8

  • eBook Packages: Springer Protocols

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