Summary
Fibronectin fibrillogenesis is a cell-mediated, step-wise process that converts soluble fibronectin into insoluble fibronectin matrix. The deposition of fibronectin fibrils occurs at specific sites on the cell surface and depends on the unfolding of the fibronectin dimer. Fibronectin matrix provides positional information for cell migration during early embryogenesis and plays an important role in cell growth, differentiation, survival, and oncogenic transformation. Here we present simple techniques, based on the use of fluorescently labeled fibronectin and species-specific antifibronectin antibodies that allow determination of the fibronectin fibril growth in conventional in vitro cell cultures and in three-dimensional matrix environment.
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Acknowledgments
Part of this work was supported by the Bulgarian National Fund for Scientific Research (Grant By-Б-1/05 and 1404/04).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Pankov, R., Momchilova, A. (2009). Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis). In: Even-Ram, S., Artym, V. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology, vol 522. Humana Press. https://doi.org/10.1007/978-1-59745-413-1_18
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DOI: https://doi.org/10.1007/978-1-59745-413-1_18
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