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Transposon Mutagenesis in Mice

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Book cover Gene Knockout Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 530))

Abstract

Understanding the functional landscape of the mammalian genome is the next big challenge of biomedical research. The completion of the first phases of the mouse and human genome projects, and expression analyses using microarray hybridization, generate critically important questions about the functional landscape and structure of the mammalian genome: how many genes, and of what type, are there; what kind of functional elements make up a properly functioning gene? One step in this process will be to create mutations in every identifiable mouse gene and analyze the resultant phenotypes. Transposons are being considered as tools to further initiatives to create a comprehensive resource of mutant mouse strains. Also, it may be possible to use transposons in true forward genetic screens in the mouse. The “Sleeping Beauty” (SB) transposon system is one such tool. Moreover, due to its tendency for local hopping, SB has been proposed as a method for regional saturation mutagenesis of the mouse genome. In this chapter, we review the tools and methods currently available to create mutant mice using in vivo, germline transposition in mice.

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Acknowledgments

This work was supported by grants R21 CA118600, R01 CA113636, and RO1 DA014764 from the National Institutes of Health (to DAL).

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Authors and Affiliations

  1. University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA

    David A. Largaespada

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  1. David A. Largaespada
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Largaespada, D.A. (2009). Transposon Mutagenesis in Mice. In: Wurst, W., Kühn, R. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 530. Humana Press. https://doi.org/10.1007/978-1-59745-471-1_20

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    • DOI: https://doi.org/10.1007/978-1-59745-471-1_20

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-26-8

    • Online ISBN: 978-1-59745-471-1

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