Abstract
Congenic strains are routinely used for positional mapping of quantitative trait loci; while conplastic strains, derived by substitution of different mitochondrial genomes on the same nuclear genetic background of inbred rodent strains, provide a way to unambiguously isolate effects of the mitochondrial genome on complex traits. Derivation of congenic or conplastic strains using a traditional backcross breeding strategy (10 backcrosses) takes more than 3 years. There are two principal strategies to speed up this process: (1) marker-assisted derivation of “speed” congenic/conplastic strains and (2) derivation of “supersonic” congenic/conplastic strains using in each backcross generation embryos obtained from 4-week-old superovulated females; thus, each backcross generation takes only 7 weeks. Both strategies could also be combined. In the current chapter, a method for derivation of “supersonic” congenic/conplastic rat strains is described.
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Acknowledments
This work was supported by grants 1P05ME791 and 1 M6837805002 from the Ministry of Education of the Czech Republic, grants 301/06/0028 and 301/08/0166 from the Grant Agency of the Czech Republic, grant IAA500110604 from the Grant Agency of the Academy of Sciences of the Czech Republic, and by the European Commission within the Sixth Framework Programme through the Integrated Project EURATools (contract no. LSHG-CT-2005-019015). M.P. is an international research scholar of the Howard Hughes Medical Institute.
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Landa, V., Zídek, V., Pravenec, M. (2010). Generation of Rat “Supersonic” Congenic/Conplastic Strains Using Superovulation and Embryo Transfer. In: Anegon, I. (eds) Rat Genomics. Methods in Molecular Biology, vol 597. Humana Press. https://doi.org/10.1007/978-1-60327-389-3_18
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DOI: https://doi.org/10.1007/978-1-60327-389-3_18
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