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μChIP: Chromatin Immunoprecipitation for Small Cell Numbers

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Chromatin Immunoprecipitation Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 567))

Abstract

Chromatin immunoprecipitation (ChIP) is a technique of choice for studying protein–DNA interactions. ChIP has been used for mapping the location of modified histones on DNA, often in relation to transcription or differentiation. Conventional ChIP protocols, however, require large number of cells, which limits the applicability of ChIP to rare cell samples. ChIP assays for small cell numbers (in the range of 10,000–100,000) have been recently reported; however, these remain lengthy. Our laboratory has elaborated fast ChIP assays suitable for small cell numbers (100–100,000) and for the immunoprecipitation of histone proteins or transcription factors under cross-linking conditions. We describe here a rapid micro (μ)ChIP assay suited for multiple parallel ChIPs from a single chromatin batch from 1,000 cells. The assay is also applicable to a single immunoprecipitation from 100 cells.

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Acknowledgments

Our work is supported by the FUGE, YFF, STAMCELLER, and STORFORSK programs of the Research Council of Norway and by the Norwegian Cancer Society.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

    John Arne Dahl & Philippe Collas

Authors
  1. John Arne Dahl
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  2. Philippe Collas
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Editors and Affiliations

  1. Institute of Basic Medical Sciences, Dept. Biochemistry, University of Oslo, Sognsvannsveien 9, Oslo, 0372, Norway

    Philippe Collas

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Dahl, J.A., Collas, P. (2009). μChIP: Chromatin Immunoprecipitation for Small Cell Numbers. In: Collas, P. (eds) Chromatin Immunoprecipitation Assays. Methods in Molecular Biology, vol 567. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-414-2_4

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  • DOI: https://doi.org/10.1007/978-1-60327-414-2_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-413-5

  • Online ISBN: 978-1-60327-414-2

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