Summary
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
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Supported by National Institutes of Health grants R01 EB006177 and U01 CA117378 and contract Y1-CN-5014.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Servoss, S., Gonzalez, R., Varnum, S., Zangar, R. (2009). High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays. In: Tainsky, M. (eds) Tumor Biomarker Discovery. Methods in Molecular Biology, vol 520. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-811-9_10
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DOI: https://doi.org/10.1007/978-1-60327-811-9_10
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