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Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display

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Immunotherapy of Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 651))

Abstract

Recombinant antibodies as therapeutics offer new opportunities for the treatment of many tumor diseases. To date, 18 antibody-based drugs are approved for cancer treatment and hundreds of anti-tumor antibodies are under development. The first clinically approved antibodies were of murine origin or human-mouse chimeric. However, since murine antibody domains are immunogenic in human patients and could result in human anti-mouse antibody (HAMA) responses, currently mainly humanized and fully human antibodies are developed for therapeutic applications.

Here, in vitro antibody selection technologies directly allow the selection of human antibodies and the corresponding genes from human antibody gene libraries. Antibody phage display is the most common way to generate human antibodies and has already yielded thousands of recombinant antibodies for research, diagnostics and therapy. Here, we describe methods for the construction of human scFv gene libraries and the antibody selection.

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Acknowledgements

We thank Stefan Dübel and Torsten Meyer for discussion and corrections on the manuscript. We gratefully acknowledge the financial support by the German ministry of education and research (BMBF, SMP “Antibody Factory” in the NGFN2 program).

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    1. Thomas Schirrmann
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    2. Michael Hust
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    1. , Center for Cell and Gene Therapy, Baylor College of Medicine, Baylor Plaza One, Houston, 77030, USA

      Patricia Yotnda

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    Schirrmann, T., Hust, M. (2010). Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display. In: Yotnda, P. (eds) Immunotherapy of Cancer. Methods in Molecular Biology, vol 651. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-786-0_11

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