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Protocols to Study the Physiology of Oral Biofilms

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 666))

Abstract

The oral cavity harbors several hundred different bacterial species that colonize both hard (teeth) and soft tissues, forming complex populations known as microbial biofilms. It is widely accepted that the phenotypic characteristics of bacteria grown in biofilms are substantially different from those grown in suspensions. Because biofilms are the natural habitat for the great majority of oral bacteria, including those contributing to oral diseases, a better understanding of the physiology of adherent populations is clearly needed to control oral microbes in health and disease. In this chapter, we use oral streptococci as examples for studying the physiology of oral biofilms.

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References

  1. Wade, W. (1999). Unculturable bacteria in oral biofilms, in Dental plaque revisited. Oral biofilms in health and disease (Newman, H. N., and Wilson, M., Eds.). Bioline, Cardiff, pp. 313–322.

    Google Scholar 

  2. Stoodley, P., Sauer, K., Davies, D. G., and Costerton, J. W. (2002) Biofilms as complex differentiated communities. Ann. Rev. Microbiol. 56, 187–209.

    Article  CAS  Google Scholar 

  3. Lemos, J. A., Abranches, J., and Burne, R. A. (2005) Responses of cariogenic streptococci to environmental stresses. Curr. Issues Mol. Biol. 7, 95–107.

    PubMed  Google Scholar 

  4. Stewart, P. S., and Franklin, M. J. (2008) Physiological heterogeneity in biofilms. Nat. Rev. Microbiol. 6, 199–210.

    Article  PubMed  CAS  Google Scholar 

  5. Loo, C. Y., Corliss, D. A., and Ganeshkumar, N. (2000) Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes. J. Bacteriol. 182, 1374–1382.

    Article  PubMed  CAS  Google Scholar 

  6. Moore, S., and Stein, W. H. (1954) A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. Biol. Chem. 211, 907–913.

    PubMed  CAS  Google Scholar 

  7. Dubois, M., Gilles, K., Hamilton, J. K., Rebers, P. A., and Smith, F. (1951) A colorimetric method for the determination of sugars. Nature. 168, 167.

    Article  PubMed  CAS  Google Scholar 

  8. Kulka, R. G. (1956) Colorimetric estimation of ketopentoses and ketohexoses. Biochem. J. 63, 542–548.

    PubMed  CAS  Google Scholar 

  9. DiPersio, J. R., Mattingly, S. J., Higgins, M. L., and Shockman, G. D. (1974) Measurement of intracellular iodophilic polysaccharide in two cariogenic strains of Streptococcus mutans by cytochemical and chemical methods. Infect. Immun. 10, 597–604.

    PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Dr. Pedro Rosalen for kindly providing images used in Fig. 7.1.

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Lemos, J.A., Abranches, J., Koo, H., Marquis, R.E., Burne, R.A. (2010). Protocols to Study the Physiology of Oral Biofilms. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 666. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-820-1_7

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  • DOI: https://doi.org/10.1007/978-1-60761-820-1_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-819-5

  • Online ISBN: 978-1-60761-820-1

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