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Segmentation of Moving Cells in Bright Field and Epi-Fluorescent Microscopic Image Sequences

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Computer Vision and Graphics (ICCVG 2010)

Part of the book series: Lecture Notes in Computer Science ((LNIP,volume 6374))

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Abstract

The monitoring of the dynamics of stem cells’ growth in culture is important in regenerative medicine. In this paper the method of cells’ images segmentation based on alternating microscopic imaging with bright field (BF) and epifluorescent (EF) images is proposed. The method consists of two principal stages: coarse segmentation of EF images followed by fine segmentation on BF ones. The latter is based on the morphological watershed from markers produced in the first stage. Due to the fact that sequence of EF is shorter than BF one, markers cannot be produced directly for all BF images. In order to create them, an additional step of morphological interpolation of markers is applied.

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Author information

Authors and Affiliations

  1. Institute of Control and Industrial Electronics, Warsaw University of Technology, ul. Koszykowa 75, 00-662, Warszawa, Poland

    Marcin Iwanowski

  2. Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, ul. Ks.Trojdena 4, 02-109, Warszawa, Poland

    Anna Korzyńska

Authors
  1. Marcin Iwanowski
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  2. Anna Korzyńska
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Editor information

Editors and Affiliations

  1. Polish-Japanese Institute of Information Technology, 86 Koszykowa St, 02-008, Warsaw, Poland

    Leonard Bolc

  2. Institute of Automatics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059, Kraków, Poland

    Ryszard Tadeusiewicz

  3. Faculty of Applied Informatics and Mathematics, Department of Informatics, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776, Warsaw, Poland

    Leszek J. Chmielewski

  4. Polish-Japanese Institute of Information Technology, 86 Koszykowa St, 02-008 Warsaw, konradw@pjwstk.edu.pl, The Silesian University of Technology, Institute of Computer Science, Akademicka 16, 44-100 Gliwice, Polan

    Konrad Wojciechowski

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Iwanowski, M., Korzyńska, A. (2010). Segmentation of Moving Cells in Bright Field and Epi-Fluorescent Microscopic Image Sequences. In: Bolc, L., Tadeusiewicz, R., Chmielewski, L.J., Wojciechowski, K. (eds) Computer Vision and Graphics. ICCVG 2010. Lecture Notes in Computer Science, vol 6374. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15910-7_46

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  • DOI: https://doi.org/10.1007/978-3-642-15910-7_46

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-15909-1

  • Online ISBN: 978-3-642-15910-7

  • eBook Packages: Computer ScienceComputer Science (R0)

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