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The cis-regulatory element CCACGTGG is involved in ABA and water-stress responses of the maize gene rab28

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Abstract

The maize gene rab28 has been identified as ABA-inducible in embryos and vegetative tissues. It is also induced by water stress in young leaves. The proximal promoter region contains the conserved cis-acting element CCACGTGG (ABRE) reported for ABA induction in other plant genes. Transient expression assays in rice protoplasts indicate that a 134 bp fragment (-194 to -60 containing the ABRE) fused to a truncated cauliflower mosaic virus promoter (35S) is sufficient to confer ABA-responsiveness upon the GUS reporter gene. Gel retardation experiments indicate that nuclear proteins from tissues in which the rab28 gene is expressed can interact specifically with this 134 bp DNA fragment. Nuclear protein extracts from embryo and water-stressed leaves generate specific complexes of different electrophoretic mobility which are stable in the presence of detergent and high salt. However, by DMS footprinting the same guanine-specific contacts with the ABRE in both the embryo and leaf binding activities were detected. These results indicate that the rab28 promoter sequence CCACGTGG is a functional ABA-responsive element, and suggest that distinct regulatory factors with apparent similar affinity for the ABRE sequence may be involved in the hormone action during embryo development and in vegetative tissues subjected to osmotic stress.

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Author notes

  1. Mark J. Guiltinan

    Present address: Department of Horticulture, The Pennsylvania State University, 16802-5807, University Park, PA, USA

Authors and Affiliations

  1. Departamento de Genética Molecular, Centro de Investigación y Desarrollo, C.S.I.C., Jorge Girona 18-26, 08034, Barcelona, Spain

    Maria Pla, Josep Vilardell, Marie Françoise Niogret & Montserrat Pagès

  2. Department of Biology, The University of North Carolina at Chapel Hill, 27599-3280, Chapel Hill, NC, USA

    Mark J. Guiltinan, William R. Marcotte & Ralph S. Quatrano

  3. Department of Biological Sciences, Clemson University, 29634-1903, Clemson, DC, USA

    William R. Marcotte

Authors
  1. Maria Pla
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  2. Josep Vilardell
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  3. Mark J. Guiltinan
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  4. William R. Marcotte
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  5. Marie Françoise Niogret
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  6. Ralph S. Quatrano
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  7. Montserrat Pagès
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Pla, M., Vilardell, J., Guiltinan, M.J. et al. The cis-regulatory element CCACGTGG is involved in ABA and water-stress responses of the maize gene rab28. Plant Mol Biol 21, 259–266 (1993). https://doi.org/10.1007/BF00019942

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  • DOI: https://doi.org/10.1007/BF00019942

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