Abstract
The iron-rich (500 μM ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved oxygen concentration. RPMI 1640 served as the control medium. Fortification of the basal medium by balanced mixtures of amino acids and vitamins showed higher positive effect than daily supplementation by glucose and glutamine. Strongly fortified medium, based on RPMI 1640, was found superior to other basal media. The viability index increased by a factor of 3.04 and the total antibody production by a factor of 2.82, relative to the control.
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References
Bursch W, Kleine L and Tenniswood M (1990) The biochemistry of cell death by apoptosis. Biochem. Cell Biol. 68: 1071–1074.
Dalili M, Sayles GD and Ollis DF (1990) Glutamine-limited batch hybridoma, growth and antibody production. Experiment and model. Biotechnol. Bioeng. 36: 74–82.
Franěk F, Dolníková J and Kovář J (1990) Monoclonal antibody production by hybridoma cultures in protein-free medium. Abstracts of the 5th European Congress on Biotechnology, Copenhagen, 1990, p. 448.
Franěk F and Dolníková J (1991) Nucleosomes occurring in protein-free hybridoma cultures. Evidence for programmed cell death. FEBS Letters 284: 285–287.
Jo E-C, Park H-J, Park J-M and Kim K-H (1990) Balanced nutrient fortification enables high-density hybridoma cell culture in batch culture. Biotechnol. Bioeng. 36: 717–722.
Kovář J and Franěk F (1987) Iron compounds at high concentrations enable hybridoma growth in a protein-free medium. Biotechnol. Letters 9: 259–264.
Luan LY, Mutharasan R and Magee WE (1987) Strategies to extend longevity of hybridomas in culture and promote yield of monoclonal antibodies. Biotechnol. Letters 9: 691–696.
Miller WM, Wilke CR and Blanch HW (1989) Transient response of hybridoma cells to nutrient additions in continuous culture: I. Glucose pulse and step changes. Biotechnol. Bioeng. 33: 477–486.
Miller WM, Wilke CR and Blanch HW (1989a) Transient response of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes. Biotechnol. Bioeng. 33: 489–499.
Ozturk SS and Palsson BO (1991) Examination of serum and bovine serum albumin as shear protective agents in agitated cultures of hybridoma cells. J. Biotechnol. 18: 13–28.
Piacentini M and Fesus L (1990) The role of transglutaminase in the formation of apoptotic bodies. Abstracts of the 20th Meeting of FEBS, Budapest 1990, p. 241.
Renard JM, Spagnoli R, Mazier C, Salles MF and Mandine E (1988) Evidence that monoclonal antibody production kinetics is related to the integral of the viable cells curve in batch systems. Biotechnol. Letters 10: 91–96.
Reuveny S, Velez D, Miller L and Macmillan JD (1986) Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors. J. Immunol. Methods 86: 61–69.
Suzuki E and Ollis DF (1990) Enhanced antibody production at slowed growth rates: Experimental demonstration and simple structured model. Biotechnol. Prog. 6: 231–236.
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Franêk, F., Dolníková, J. Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: Effect of nutrient concentration. Cytotechnology 7, 33–38 (1991). https://doi.org/10.1007/BF00135636
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DOI: https://doi.org/10.1007/BF00135636