Skip to main content
SpringerLink
Log in

Vinculin, talin and focal adhesions

  • News and Views
  • Published:
Journal of Muscle Research & Cell Motility Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • ADAMSONE. D., BARIBAULTH. & KEMLERR. (1990) Altered uvomorulin expression in a non-compacting mutant cell line of F9 embryonal carcinoma cells. Dev. Biol. 138, 338–47.

    Google Scholar 

  • BARSTEADR. J. & WATERSTONR. H. (1989) The basal component of the nematode dense-body is vinculin. J. Biol. Chem. 264, 10177–85.

    Google Scholar 

  • BARSTEADR. J. & WATERSTONR. H. (1991) Vinculin is essential for muscle function in the nematode. J. Cell Biol. 114, 1715–24.

    Google Scholar 

  • BECKERLEM. C., BURRIDGEK., DERMATINOG. N. & CROALLD. E. (1987) Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesions. Cell 51, 569–77.

    Google Scholar 

  • BURRIDGEK. & MANGEATP. (1984) An interaction between vinculin and talin. Nature 308, 744–6.

    Google Scholar 

  • COLLJ. L., BEN-ZE'EVA., EZZELLR. M., RODRIGUEZ FERNANDEZJ. L., BARIBAULTH., OSHIMAR. G. & ADAMSONE. D. (1995) Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion. Proc. Natl. Acad. Sci. USA 92, 9161–5.

    Google Scholar 

  • COUTUM. D. & CRAIGS. W. (1988) cDNA-derived sequence of chicken embryo vinculin. Proc. Natl. Acad. Sci. USA 85, 8535–9.

    Google Scholar 

  • CRAWFORDA. W., MICHELSENJ. W. & BECKERLEM. C. (1992) An interaction between zyxin and alpha-actinin. J. Cell Biol. 116, 1381–93.

    Google Scholar 

  • DAHLS. C. & GRABELL. B. (1989) Integrin phosphorylation is modulated during the differentiation of F-9 teratocarcinoma stem cells. J. Cell Biol. 108, 183–90.

    Google Scholar 

  • FRITZM., ZIMMERMANNR., BAERMANNM. & GAUBH. E. (1993) Actin binding to lipid-inserted alpha-actinin. Biophysical J. 65, 1878–85.

    Google Scholar 

  • FUKAMIK., ENDOT., IMAMURAM. & TAKENAWAJ. (1994) Alpha-actinin and vinculin are PIP2-binding proteins involved in signaling by tyrosine kinase. J. Biol. Chem. 269, 1518–22.

    Google Scholar 

  • GEIGERB. & GINSBERGD. (1991) The cytoplasmic domain of adherens-type junctions. Cell Mot. Cytoskel. 20, 1–6.

    Google Scholar 

  • GILMOREA. P., WOODC., OHANIANV., JACKSONP., PATELB., REESD. J. G., HYNESR. O. & CRITCHLEYD. R. (1993) The cytoskeletal protein talin contains at least two distinct vinculin binding domains. J. Cell Biol. 122, 337–47.

    Google Scholar 

  • GLENNEYJ. R. & ZOKASL. (1989) Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton. J. Cell Biol. 108, 2401–8.

    Google Scholar 

  • GOLDMANNW. H. & ISENBERGG. (1991) Kinetic determination of talin-actin binding. Biochem. Biophys. Res. Comm. 178, 718–23.

    Google Scholar 

  • GOLDMANNW. H., NIGGLIV., KAUFMANNS. & ISENBERGG. (1992) Probing actin and liposome interaction of talin and talin-vinculin complexes: a kinetic thermodynamic and lipid labeling study. Biochemistry 31, 7665–71.

    Google Scholar 

  • GOLDMANNW. H., BREMERA., HAENERM., AEBIU. & ISENBERGG. (1994) Native talin is a dumbbell-shaped homodimer when it interacts with actin. J. Struct. Biol. 112, 3–10.

    Google Scholar 

  • GOLDMANNW. H., SCHINDLM., CARDOZOT. J. & EZZELLR. M. (1995) Motility of vinculin-deficient F9 embryonic carcinoma cells analyzed by video, laser confocal, and reflection interference contrast microscopy. Exp. Cell Res. 221, 311–19.

    Google Scholar 

  • GROVERA., ROSENSTRAUSM. J., STERMANB., SNOOKM. E. & ADAMSONE. D. (1987) An adhesion-defective variant of F9 embryonal carcinoma cells fails to differentiate into visceral endoderm. Dev. Biol. 119, 1–11.

    Google Scholar 

  • HORWITZA., DUGGANE., BUCKC., BECKERLEM. C. & BURRIDGEK. (1986) Interaction of plasma membrane fibronectin receptor with talin: a transmembrane linkage. Nature 320, 531–3.

    Google Scholar 

  • HYNESR. O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69, 11–25.

    Google Scholar 

  • ISENBERGG. & GOLDMANNW. H. (1992) Actin-membrane coupling: a role for talin. J. Muscle Res. Cell Mot. 13, 587–9.

    Google Scholar 

  • JAKENS., LEACHK. & KLAUCKT. (1989) Association of type 3 protein kinase C with focal contacts in rat embryo fibroblasts. J. Cell Biol. 109, 697–704.

    Google Scholar 

  • JOHNSONR. P. & CRAIGS. W. (1994) An intramolecular association between the head and tail domains of vinculin modulates talin binding. J. Biol. Chem. 269, 12611–19.

    Google Scholar 

  • JOHNSONR. P. & CRAIGS. W. (1995) F-actin binding site masked by the intramolecular association of vinculin head and tail domains. Nature 373, 261–4.

    Google Scholar 

  • KAUFMANNS., KAESJ., GOLDMANNW. H., SACKMANNE. & ISENBERGG. (1992) Talin anchors and nucleates actin filaments at lipid membranes: a direct demonstration. FEBS Lett. 314, 203–5.

    Google Scholar 

  • MAHERP. A., PASQUALEE. B., WANGJ. Y. J. & SINGERS. J. (1985) Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells. Proc. Natl. Acad. Sci. USA 82, 6576–80.

    Google Scholar 

  • MCLACHLANA. D., STEWARTM., HYNESR. O. & REESD. J. G. (1994) Analysis of repeated motifs in the talin rod. J. Mol. Biol. 235, 1278–90.

    Google Scholar 

  • MENKELA. R., KROEMKERM., BUBECKP., RONSIEKM., NIKOLAIG. & JOCKUSCHB. M. (1994) Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin. J. Cell Biol. 126, 1231–40.

    Google Scholar 

  • MILAML. M. (1985) Electron microscopy of rotary shadowed vinculin and vinculin complexes. J. Mol. Biol. 184, 543–5.

    Google Scholar 

  • MOLONYL. & BURRIDGEK. (1985) Molecular shape and self-association of vinculin and metavinculin. J. Cell Biochem. 29, 31–6.

    Google Scholar 

  • NIGGLIV. & BURGERM. M. (1987) Interaction of the cytoskeleton with the plasma membrane. J. Membrane Biol. 100, 97–121.

    Google Scholar 

  • NIGGLIV., KAUFMANNS., GOLDMANNW. H., WEBERT., & ISENBERGG. (1994) Identification of functional domains in the cytoskeletal protein talin. Eur. J. Biochem. 224, 951–7.

    Google Scholar 

  • NUCKOLLSG. H., ROMERL. H. & BURRIDGEK. (1992) Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts. J. Cell Sci. 102, 753–62.

    Google Scholar 

  • PAVALKOF., OTEYC. & BURRIDGEK. (1989) Identification of a filamin isoform enriched at the ends of stress fibers in chicken embryo fibroblasts. J. Cell Sci. 94, 109–18.

    Google Scholar 

  • PRICEG. J., JONESP., DAVISONM. D., PATELB., EPERONI. C. & CRITCHLEYD. R. (1987) Isolation and characterization of a vinculin cDNA from chick-embryo fibroblasts. Biochem. J. 245, 595–603.

    Google Scholar 

  • PRICEG. J., JONESP., DAVISONM. D., PATELB., BENDORIR., GEIGERB. & CRITCHLEYD. R. (1989) Primary sequence and domain structure of chicken vinculin. Biochem. J. 259, 453–61.

    Google Scholar 

  • QWARNSTROME. E., MACFARLANES. A., PAGER. C. & DOWERS. K. (1991) Interleukin-1 beta induces rapid phosphorylation and redistribution of talin: a possible mechanism for modulation of fibroblast focal adhesions. Proc. Natl. Acad. Sci. USA 88, 1232–6.

    Google Scholar 

  • RODRIGUEZ FERNANDEZJ. L., GEIGERB., SALOMOND. & BEN-ZE'EVA. (1992) Overexpression of vinculin suppresses cell motility in BALB/c 3T3 cells. Cell Mot. Cytoskel. 22, 127–34.

    Google Scholar 

  • RODRIGUEZ FERNANDEZJ. L., GEIGERB., SALOMOND. & B-ZE'EVA. (1993) Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells. J. Cell Biol. 122, 1285–94.

    Google Scholar 

  • ROMERL. H., BURRIDGEK. & TURNERC. E. (1992) Signaling between the extracellular matrix and the cytoskeleton: tyrosine phosphorylation and focal adhesion assembly. Cold Spring Harb. Symp. Quant. Biol. 57, 193–202.

    Google Scholar 

  • SAMUELSM., EZZELLR. M., CARDOZOT. J., CRITCHLEYD. R., COLLJ. L. & ADAMSONE. D. (1993) Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell. J. Cell Biol. 121, 909–21.

    Google Scholar 

  • SEFTONB., HUNTERT., BALLE. & SINGERS. J. (1981) Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus. Cell 24, 165–7.

    Google Scholar 

  • TEMPELM., GOLDMANNW. H., ISENBERGG. & SACKMANNE. (1995) Interaction of the 47-kDa talin fragment and the 32-kDa vinculin fragment with acidic phospholipids: a computer analysis. Biophys. J. 69, 228–41.

    Google Scholar 

  • TURNERC. E. & BURRIDGEK. (1991) Transmembrane molecular assemblies in cell-extracellular matrix interactions. Curr. Opin. Cell Biol. 3, 849–53.

    Google Scholar 

  • TURNERC. E., PAVALKOF. M. & BURRIDGEK. (1989) The role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity. J. Biol. Chem. 264, 11938–44.

    Google Scholar 

  • TURNERC. E., GLENNEYJrJ. R. & BURRIDGEK. (1990) Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111, 1059–68.

    Google Scholar 

  • VARNUM-FINNEYB. & REICHARDTL. F. (1994) Vinculin-deficient PC12 cell lines extend unstable lamellipodia and filopodia and have a reduced rate of neurite outgrowth. J. Cell Biol. 127, 1071–84.

    Google Scholar 

  • VOLBERGT., GEIGERB., KAMZ., PANKOVR., SIMCHAI., SABANAYH., COLLL. J., ADAMSONE. D. & BEN-ZE'EVA. (1995) Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption. J. Cell Sci. 108, 2253–60.

    Google Scholar 

  • WACHSSTOCKD. H., WILKINSJ. A. & LINS. (1987) Specific interaction of vinculin with alpha-actinin. Biochem. Biophys. Res. Comm. 146, 554–60.

    Google Scholar 

  • WELLERP. A., OGRYZKOE. P., CORBENE. B., ZHIDKOVAN. I., PATELB., PRICEG. J., SPURRN. K., KOTELIANSKYV. E. & CRITCHLEYD. R. (1990) Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc. Natl. Acad. Sci. USA 87, 5667–71.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Surgery Research Laboratory, Massachusetts General Hospital, Harvard Medical School, 02129, Charlestown, MA

    Wolfgang H. Goldmann & Robert M. Ezzell

  2. Burnham Foundation, 10901, N. Torrey Pines Road, 92037, La Jolla, CA, USA

    Eileen D. Adamson

  3. Department of Pathology, University of Bern, CH-3010, Bern, Switzerland

    Verena Niggli

  4. Technical University of Munich, Biophysics E22, D-85747, Garching, Germany

    Gerhard Isenberg

Authors
  1. Wolfgang H. Goldmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert M. Ezzell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eileen D. Adamson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Verena Niggli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gerhard Isenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goldmann, W.H., Ezzell, R.M., Adamson, E.D. et al. Vinculin, talin and focal adhesions. J Muscle Res Cell Motil 17, 1–5 (1996). https://doi.org/10.1007/BF00140319

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00140319

Keywords

Search

Navigation