Abstract
Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Al-Rubeai MA, Rookes S and Emery AN (1989) Flow cytometric studies during synchronous and asynchronous suspension cultures of hybridoma cells. In: Spier RE, Griffiths JB, Stephenne J and Crooy PJ (eds.) Advances in Animal Cell Biology and Technology for Bioprocesses. Butterworths, London, pp. 241–245.
Al-Rubeai MA and Emery AN (1989) Monoclonal antibody accumulation and release observed in sub-cellular structures in synchronous and asynchronous hybridoma culture. Cytotechnology S9 (Abstract).
Biberfield P (1971) Uropod formation in phytohaemagglutinin (PHA) stimulated lymphocytes. Expl. Cell Res. 66: 433–445.
Bienkowski RS (1983) Intracellular degradation of newly synthesised secretory proteins. Biochem. J. 214: 1–10.
Doorbar J, Evans HS, Coneron J, Crawford LV and Gallimore PH (1988) Analysis of HPV-1 E4 gene expression using epitope defined antibodies. EMBO J. 7: 825–832.
Sheldrake AR (1974) The ageing, growth and death of cells. Nature 250: 381–385.
Wyllie AH (1981) Cell death: a new classification separating apoptosis from necrosis. In: Cell death in biology and pathology. Bowen ID and Lockshin RA (eds.) Chapman and Hall, London, pp. 9–34.
Wyllie AH, Duvall E and Blow JJ (1984) Intracellular mechanisms in cell death in normal and pathological tissues. In: Cell Ageing and Cell Death. Davies I and Siegg DC (eds.) Cambridge University Press, Cambridge, pp. 269–294.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Al-Rubeai, M., Mills, D. & Emery, A.N. Electron microscopy of hybridoma cells with special regard to monoclonal antibody production. Cytotechnology 4, 13–28 (1990). https://doi.org/10.1007/BF00148807
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00148807