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Optimization of amino acid supplements for heterologous protein secretion in Saccharomyces cerevisiae

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Biotechnology Techniques

Summary

The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosaccharomyces pombe acid phosphatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an 8-fold increase in total phosphatase secretion, and a 70% increase in specific phosphatase secretion.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Illinois, 61801, Urbana, IL, USA

    K. D. Wittrup & V. Benig

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  1. K. D. Wittrup
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  2. V. Benig
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Wittrup, K.D., Benig, V. Optimization of amino acid supplements for heterologous protein secretion in Saccharomyces cerevisiae . Biotechnol Tech 8, 161–166 (1994). https://doi.org/10.1007/BF00161582

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  • DOI: https://doi.org/10.1007/BF00161582

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