Abstract
Wild-type Aspergillus niger NRRL-3 was transformed with multiple copies of the glucose oxidase structural gene (god). The gene was placed under the control of the gpd A promoter of A. nidulans. For more efficient secretion the α-amylase signal peptide from A oryzae was inserted in front of god. Compared to the wild type, the recombinant strain NRRL-3 (GOD3-18) produced up to four times more extracellular glucose oxidase under identical culture conditions. Addition of yeast extract (2 g l−1) to a mineral salts medium containing only glucose as carbon source increased volumetric and specific extracellular glucose oxidase activities by 130% and 50% respectively. With the same medium composition and inoculum size, volumetric and specific extracellular glucose oxidase activities increased more than ten times in bioreactor cultivations compared to shake-flask cultures.
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Hellmuth, K., Pluschkell, S., Jung, JK. et al. Optimization of glucose oxidase production by Aspergillus niger using genetic-and process-engineering techniques. Appl Microbiol Biotechnol 43, 978–984 (1995). https://doi.org/10.1007/BF00166912
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DOI: https://doi.org/10.1007/BF00166912