Summary
A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.
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Gaffney, E.V. A cell line (HBL-100) established from human breast milk. Cell Tissue Res. 227, 563–568 (1982). https://doi.org/10.1007/BF00204786
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DOI: https://doi.org/10.1007/BF00204786