Skip to main content
Log in

RFLP analysis of soybean seed protein and oil content

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The objectives of this study were to present an expanded soybean RFLP map and to identify quantitative trait loci (QTL) in soybean [Glycine max (L.) Merr.] for seed protein and oil content. The study population was formed from a cross between a G. max experimental line (A81-356022) and a G. soja Sieb. and Zucc. plant introduction (PI 468916). A total of 252 markers was mapped in the population, forming 31 linkage groups. Protein and oil content were measured on seed harvested from a replicated trial of 60 F2-derived lines in the F3 generation (F2∶3 lines). Each F2∶3 line was genotyped with 243 RFLP, five isozyme, one storage protein, and three morphological markers. Significant (P<0.01) associations were found between the segregation of markers and seed protein and oil content. Segregation of individual markers explained up to 43% of the total variation for specific traits. All G. max alleles at significant loci for oil content were associated with greater oil content than G. soja alleles. All G. soja alleles at significant loci for protein content were associated with greater protein content than G. max alleles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burton JW (1985) Breeding soybeans for improved protein quantity and quality. In: Shibles R (ed) Proc 3rd World Soybean Res. Conf. Westview Press, Boulder/CO, pp 361–367

    Google Scholar 

  • Dickinson CD, Floener LA, Lilley GG, Nielsen NC (1987) Self-assembly of proglycinin and hybrid proglycinin synthesized in vitro from cDNA. Proc Natl Acad Sci USA 84:5525–5529

    Google Scholar 

  • Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125

    Google Scholar 

  • Fehr WR (1987) Principles of cultivar development. Macmillan, New York

    Google Scholar 

  • Graef GL, Fehr WR, Cianzio SR (1989) Relation of isozyme genotypes to quantitative characters in soybean. Crop Sci 29:683–688

    Google Scholar 

  • Keim P, Shoemaker RC (1988) Construction of a random recombinant DNA library that is primarily single copy sequences. Soybean Genet Newsl 15:147–148

    Google Scholar 

  • Keim P, Shoemaker RC, Palmer RG (1989) Restriction fragment length polymorphism diversity in soybean. Theor Appl Genet 77:786–797

    Google Scholar 

  • Keim P, Diers BW, Shoemaker RC (1990a) Genetic analysis of soybean hard seededness with molecular markers. Theor Appl Genet 79:465–469

    Google Scholar 

  • Keim P, Diers BW, Olson T, Shoemaker RC (1990b) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742

    Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Google Scholar 

  • Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73:350–356

    Google Scholar 

  • Palmer RG, Kilen TC (1987) Qualitative genetics and cytogenetics. In: Wilcox JR (ed) Soybean: improvement, production and uses, 2nd edn. Agronomy 16:23–48

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Google Scholar 

  • Rennie BD, Thorpe ML, Beversdorf WD (1989) A procedural manual for the detection and identification of soybean [Glycine max (L.) Merr.] isozymes using the starch gel electrophoretic system. Dept Crop Sci Tech Bull. University of Guelph, Ontario, Canada

    Google Scholar 

  • Smith KJ, Huyser W (1987) World distribution and significance of soybean. In: Wilcox JR (ed) Soybeans: improvement, production, and uses, 2nd edn. Agronomy 16:23–48

  • Suarez JC (1989) Association between isozyme genotypes with quantitative traits in soybean. PhD thesis, Iowa State University, Ames/IA (Diss Abstr 89–20190)

    Google Scholar 

  • Wilcox JR (1985) Breeding soybeans for improved oil quantity and quality. In: Shibles R (ed) Proc 3rd World Soybean Res. Con. Westview Press, Boulder/CO, pp 380–386

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by A. L. Kahler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Diers, B.W., Keim, P., Fehr, W.R. et al. RFLP analysis of soybean seed protein and oil content. Theoret. Appl. Genetics 83, 608–612 (1992). https://doi.org/10.1007/BF00226905

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226905

Key words

Navigation