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A simple dynamic method for on-line and off-line determination of kLa during cultivation of animal cells

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Biotechnology Techniques

Summary

A new, fast method is described to determine kLa either off-line, or on-line during animal-cell cultivation. Since it does not need the equilibrium concentration of oxygen in the liquid phase (C*), it is not required to await a new steady state. Furthermore, the results do not depend on the calibration value of the dissolved-oxygen probe. The method yielded accurate values for kLa, both for an oxygen-consuming and a non-consuming system.

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Abbreviations

C L :

Dissolved-oxygen concentration [mol·m-3]

C * :

C L in equilibrium with the oxygen concentration in the gas phase [mol·m-3]

C L,∞ :

Equilibrium oxygen concentration at stationary conditions [mol·m-3]

kLa:

Volumetric oxygen transfer coefficient [s-1]

r:

Specific oxygen consumption of biomass [mol·cell-1·s-1]

X:

Cell concentration [cells·m-3]

t:

Time [s]

ν:

Noise of dissolved-oxygen probe [mol·m-3]

ɛ:

Absolute error of kLa-measurement [s-1]

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Authors and Affiliations

  1. National Institute of Public Health and Environmental Protection, Laboratory for Product and Process Development, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands

    R. C. Dorresteijn & E. C. Beuvery

  2. Department of Food Science, Food and Bioprocess Engineering Group, Wageningen Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands

    R. C. Dorresteijn, C. D. de Gooijer & J. Tramper

Authors
  1. R. C. Dorresteijn
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  2. C. D. de Gooijer
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  3. J. Tramper
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  4. E. C. Beuvery
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Dorresteijn, R.C., de Gooijer, C.D., Tramper, J. et al. A simple dynamic method for on-line and off-line determination of kLa during cultivation of animal cells. Biotechnol Tech 8, 675–680 (1994). https://doi.org/10.1007/BF00241697

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  • DOI: https://doi.org/10.1007/BF00241697

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