Summary
The fimD gene of Escherichia coli K12 was shown to be necessary for surface localization of type 1 fimbriae, since deletion of the gene resulted in a virtually bald phenotype. The FimD protein was found to be located in the outer membrane. Expressed alone, this protein had a very deleterious effect on cell growth. The DNA sequence of the fimD gene was determined; the corresponding amino acid sequence of the FimD protein was compared with those of the PapC and FaeD proteins. A deletion derivative of FimD gave clues as to which parts of the protein were necessary for outer membrane integration.
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Communicated by J. Lengeler
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Klemm, P., Christiansen, G. The fimD gene required for cell surface localization of Escherichia coli type 1 fimbriae. Molec. Gen. Genet. 220, 334–338 (1990). https://doi.org/10.1007/BF00260505
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DOI: https://doi.org/10.1007/BF00260505