Abstract
A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems.
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Meadows, M.G., Potrykus, I. Hoechst 33258 as a vital stain for plant cell protoplasts. Plant Cell Reports 1, 77–79 (1981). https://doi.org/10.1007/BF00269279
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DOI: https://doi.org/10.1007/BF00269279