Skip to main content
Log in

DNA hybridization analyses of a Gossypium allotetmploid and two closely related diploid species

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The DNAs of two diploid species of Gossypium, G. herbaceum var. africanum (A1 genome) and G. raimondii (D5 genome), and the allotetraploid species, G. hirsutum (Ah and Dh genomes), were characterized by kinetic analyses of single copy and repetitive sequences. Estimated haploid genome sizes of A1 and D5 were 1.04 pg and 0.68 pg, respectively, in approximate agreement with cytological observations that A genome chromosomes are about twice the size of D genome chromosomes. This differences in genome size was accounted for entirely by differences in the major repetitive fraction (0.56 pg versus 0.20 pg), as single copy fractions of the two genomes were essentially identical (0.41 pg for A1 and 0.43 pg for D5). Kinetic analyses and thermal denaturation measurements of single copy duplexes from reciprocal intergenomic hybridizations showed considerable sequence similarity between A1 and D5 genomes (77% duplex formation with an average thermal depression of 6 °C). Moreover, little sequence divergence was detectable between diploid single copy sequences and their corresponding genomes in the allotetraploid, consistent with previous chromosome pairing observations in interspecific F1 hybrids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bonner TI, Brenner DT, Neufeld BR, Britten RJ (1973) Reduction in the rate of reassociation by sequence divergence. J Mol Biol 81:123–135

    Google Scholar 

  • Britten RJ (1986) Rates of DNA sequence evolution differ between taxonomic groups. Science 231:1393–1398

    Google Scholar 

  • Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. Methods Enzymol 29:363–418

    Google Scholar 

  • Britten RJ, Cetta A, Davidson EH (1978) The single copy DNA sequence polymorphism of the sea urchin Strongylocentrotus purpuratus. Cell 15:1175–1186

    Google Scholar 

  • Davidson EH, Hough BR, Amenson CS, Britten RJ (1973) General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J Mol Biol 77:1–23

    Google Scholar 

  • Edwards GA, Endrizzi JE, Stein R (1974) Genome DNA content and chromosome organization in Gossypium. Chromosoma 47:309–326

    Google Scholar 

  • Endrizzi JE, Turcotte EL, Kohel RJ (1985) Genetics, cytology, and evolution of Gossypium. Adv Genet 23:271–375

    Google Scholar 

  • Ergle DR, Katterman FRH, Richmond TR (1964) Aspects of nucleic acid composition of Gossypium. Plant Physiol 39:145–150

    Google Scholar 

  • Fryxell PA (1979) The natural history of the cotton tribe. Texas A&M University Press, p XVIII-245

    Google Scholar 

  • Hutchinson J, Narayan RKJ, Rees H (1980) Constraints upon the composition of supplementary DNA. Chromosoma 78:137–145

    Google Scholar 

  • Kadir ZBA (1976) DNA evolution in the genus Gossypium. Chromosoma 56:85–94

    Google Scholar 

  • Katterman FRH, Ergle DR (1970) A study of qualitative variations of nucleic acids in Gossypium. Phytochemistry 9:2007–2010

    Google Scholar 

  • Katterman FRH, Shattuck VI (1983) An effective method of DNA isolation form mature leaves of Gossypium species that contain large amounts of phenolic terponoids and tannins. Prep Biochem 13:347–359

    Google Scholar 

  • Kohel RJ, Richmond TR, Lewis CF (1970) Texas Marker 1. Description of a genetic standard for Gossypium hirsutum L. Crop Sci 10:670–671

    Google Scholar 

  • McCarty Jr. KS, Vollmer RT, McCarty KS (1974) Improved computer program data for the resolution and fractionation of macromolecules by isokinetic sucrose density gradient sedimentation. Ann Biochem 161:165–183

    Google Scholar 

  • Murphy RF, Pearson WR, Bonner J (1979) Computer programs for analysis of nucleic acid hybridization, thermal denaturation, and gel electrophoresis data. Nucleic Acids Res 6:3911–3920

    Google Scholar 

  • Skovsted A (1934) Cytological studies in cotton II. Two interspecific hybrids between Asiatic and New World cottons. J Genet 28:407–424

    Google Scholar 

  • Studier FW (1965) Sedimentation studies of the size and shape of DNA. J Mol Biol 11:373–390

    Google Scholar 

  • Walbot V, Dure III LS (1976) Development biochemistry of cotton seed embryogenesis and germination. VII. Characterization of the cotton genome. J Mol Biol 101:503–536

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Salamini

Journal paper No. 4461 of the Arizona Agricultural Experiment Station

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geever, R.F., Katterman, F.R.H. & Endrizzi, J.E. DNA hybridization analyses of a Gossypium allotetmploid and two closely related diploid species. Theoret. Appl. Genetics 77, 553–559 (1989). https://doi.org/10.1007/BF00274278

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00274278

Key words

Navigation