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Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae

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Abstract

The Saccharomyces cerevisiae YAP2 gene encoding an AP-1-like transcriptional activator protein was cloned by selection for genes that confer pleiotropic drug resistance when present in high copy number. The novel YAP2 gene encodes a protein of 45827 daltons and is homologous in part to a known transcriptional activator protein encoded by YAP1/PDR4/SNQ3/PAR1. Homology was found only in both terminal regions. The N-terminal portion contains a region rich in basic amino acids, followed by a “leucine zipper” motif. Overexpression of YAP2 led to the induction of expression of an AP-1 recognition element (ARE)-dependent promoter. The yap1 disruptant has been shown to be sensitive to H2O2. In this study, we demonstrated that the yap1 disruptant is also unable to grow in medium containing 150 μM cadmium, whereas the yap2 disruptant exhibited no significant phenotypes. However, YAP2 in high copy number did suppress cadmium sensitivity, but not H2O2 sensitivity of the yap1 disruptant. YAP1 was able to mediate both cadmium- and H2O2-induced transcriptional activation of an ARE-dependent promoter. A high-copy-number plasmid bearing YAP2 mediated cadmium-induced transcriptional activation of this promoter. The inductions were prevented by the antioxidant N-acetyl-l-cysteine.

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References

  • Amati B, Dalton S, Brooks MW, Littlewood TD, Evan GI, Land H (1992) Transcriptional activation by the human c-Myc onco-protein in yeast requires interaction with Max. Nature 359:423–426

    Google Scholar 

  • Berstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24

    Google Scholar 

  • Boyle WJ, Smeal T, Defize LHK, Angel P, Woodgett JR, Karin M, Hunter T (1991) Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity. Cell 64:573–584

    Google Scholar 

  • Devary Y, Gottlieb RA, Lau LF, Karin M (1991) Rapid and preferential activation of the c-jun gene during the mammalian UV response. Mol Cell Biol 11:2804–2811

    Google Scholar 

  • Devary Y, Gottlieb RA, Smeal T, Karin M (1992) The mammalian ultraviolet response is triggered by activation of Src tyrosine kinases. Cell 71:1081–1091

    Google Scholar 

  • Guarente L (1984) Yeast promoters: positive and negative elements. Cell 36:799–800

    Google Scholar 

  • Harshman KD, Moye WS, Parker CS (1988) Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4. Cell 53:321–330

    Google Scholar 

  • Hertle K, Haase E, Brendel M (1991) The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals. Curt Genet 19:429–433

    Google Scholar 

  • Hunter T, Karin M (1992) The regulation of transcription by phosphorylation. Cell 70:375–387

    Google Scholar 

  • Hussain M, Lenard J (1991) Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number. Gene 101:149–152

    Google Scholar 

  • Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163–168

    Google Scholar 

  • Johnston M, Davis RW (1984) Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol Cell Biol 4:1440–1445

    Google Scholar 

  • Leevers SJ, Marshall CJ (1992) MAP kinase regulation — the oncegene connection. Trends Cell Biol 2:283–286

    Google Scholar 

  • Lewin B (1990) Commitment and activation at Pol II promoters: a tail of protein-protein interactions. Cell 61:1161–1164

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • McKnight S, Tjian R (1986) Transcriptional selectivity of viral genes in mammalian cells. Cell 46:795–805

    Google Scholar 

  • Meyer M, Schreck R, Baeuerle PA (1993) H2O2 and antioxidants have opposite effects on activation of NF-κB and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. EMBO J 12:2005–2015

    Google Scholar 

  • Miller JH (1992) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Mitchell PJ, Tjian R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245:371–378

    CAS  PubMed  Google Scholar 

  • Moye-Rowley WS, Harshman KD, Parker CS (1989) Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins. Genes Dev 3:283–292

    Google Scholar 

  • Pulverer BJ, Kyriakis JM, Avruch J, Nikolakaki E, Woodgett JR (1991) Phosphorylation of c-jun mediated by MAP kinases. Nature 353:670–674

    Google Scholar 

  • Roederer M, Staal FJT, Raju PA, Ela SW, Herzenberg LA, Herzenberg LA (1990) Cytokine-stimulated human immunodeficiency virus replication is inhibited by N-acetyl-l-cysteine. Proc Natl Acad Sci USA 87:4884–4888

    Google Scholar 

  • Rothstein R (1983) One-step gene disruption in yeast. Methods Enzymol 101:202–211

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 83:5463–5467

    Google Scholar 

  • Schnell N, Entian KD (1991) Identification and characterization of a Saccharomyces cerevisiae gene (PAR1) conferring resistance to iron chelators. Eur J Biochem 200:487–493

    Google Scholar 

  • Schnell N, Krems B, Entian KD (1992) PAR1 (YAPI/SNQ3) gene of Saccharomyces cerevisiae, a c-jun homologue, is involved in oxygen metabolism. Curr Genet 21:269–273

    Google Scholar 

  • Schreck R, Rieber P, Baeuerle PA (1991) Reactive oxygen intermediates as apparently widely used measengers in the activation of the NF-κB transcription factor and HIV-1. EMBO J 10:2247–2258

    Google Scholar 

  • Staal FJ, Roederer M, Herzenberg LA, Herzenberg LA (1990) Intracellular thiols regulate activation of nuclear factor κCB and transcription of human immunodeficiency virus. Proc Natl Acad Sci USA 87:9943–9947

    Google Scholar 

  • Toda T, Shimanuki M, Yanagida M (1991) Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases. Genes Dev 5:60–73

    Google Scholar 

  • Yamashita I, Fukui S (1983) Molecular cloning of a glucoamylase producing gene in the yeast Saccharomyces. Agric Biol Chem 47:2689–2692

    Google Scholar 

  • Yamashita I, Nakamura M, Fukui S (1987) Gene fusion is a possible mechanism underlying the evolution of STA1. J Bacteriol 169:2142–2149

    Google Scholar 

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Communicated by C.P. Hollenberg

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Hirata, D., Yano, K. & Miyakawa, T. Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae . Molec. Gen. Genet. 242, 250–256 (1994). https://doi.org/10.1007/BF00280413

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